Intact carboxysomes in a cyanobacterial cell visualized by Hilbert differential contrast transmission electron microscopy
Intact carboxysomes in a cyanobacterial cell visualized by Hilbert differential contrast transmission electron microscopy
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DOI：
10.1128/jb.188.2.805-808.2006
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发表时间：
2006-01-01
影响因子：
3.2
通讯作者：
Nakamoto, H
中科院分区：
文献类型：
--
作者：
Kaneko, Y;Danev, R;Nakamoto, H
冰冻蓝藻聚球藻全细胞中的羧基体。用新近研制的希尔伯特差示对比透射电子显微镜对PCC7942菌株进行了观察。在破裂的细胞中，羧基小体的结构细节尤其清晰。新的电子显微镜显示了羧基体中核酮糖-1，5-二磷酸羧基酶/加氧酶分子的准晶阵列，与传统的细胞超薄切片的透射电子显微镜相比，形成了更好的对比。羧体被一层5到6纳米厚的单层外壳包围，外壳由排列整齐的球形颗粒组成。
Carboxysomes in rapidly frozen ice-embedded whole cells of the cyanobacterium Synechococcus sp. strain PCC 7942 were visualized by the recently developed Hilbert differential contrast transmission electron microscope. Structural details of carboxysomes were especially clearly visualized in the ruptured cells. The novel electron microscopy exhibited the paracrystalline arrays of molecules of the enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase in the carboxysomes in much better contrast than conventional transmission electron microscopy with ultrathin sections of cells. The carboxysome was surrounded by a 5- to 6-nm-thick monolayer shell which consisted of orderly arrays of globular particles.