Sexual isolation of male moths explained by a single pheromone response QTL containing four receptor genes
Sexual isolation of male moths explained by a single pheromone response QTL containing four receptor genes
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DOI：
10.1073/pnas.0910945107
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发表时间：
2010-05-11
影响因子：
11.1
通讯作者：
Vickers, Neil J.
中科院分区：
文献类型：
--
作者：
Gould, Fred;Estock, Marie;Vickers, Neil J.
蛾类的远距离性交流一直吸引着生物学家，因为复杂、精确的雌性信息素信号和雄性对特定信息素分子的极端敏感性。在确定一些参与雌性信息素产生和雄性反应的基因方面已经取得了进展。然而，我们缺乏有关这些择偶机制进化多样化所涉及的遗传变化的信息，而这些遗传变化对理解飞蛾和其他类群的物种形成至关重要。我们使用了一个组合的数量性状位点（QTL）和候选基因的方法来确定性隔离的遗传结构在两个同属蛾，Heliothis subflexa和Heliothis virescens的男性。我们报告的行为和神经生理学的证据表明，差异男性的反应，以三个女性产生的化学品（Z9-14：Ald，Z9-16：Ald，Z11-16：OAc），保持这些物种的性隔离都是由一个单一的QTL包含至少四个气味受体基因控制。信息素受体的差异可以控制H. subflexa和H. virescens对Z9-16：Ald和Z9-14：Ald的反应。然而，中枢而不是外周水平控制的积极和消极的反应H。subflexa和H. virescens对Z11-16：0Ac的抗性。这些受体基因的紧密连锁表明，突变改变男性复杂的混合物的反应可以保持连锁不平衡，并可能影响物种形成过程。其他候选基因，如信息素结合蛋白的编码没有映射到这个QTL，但有一些遗传证据的QTL响应Z11-16：OH与感觉神经元膜蛋白基因。
Long distance sexual communication in moths has fascinated biologists because of the complex, precise female pheromone signals and the extreme sensitivity of males to specific pheromone molecules. Progress has been made in identifying some genes involved in female pheromone production and in male response. However, we have lacked information on the genetic changes involved in evolutionary diversification of these mate-finding mechanisms that is critical to understanding speciation in moths and other taxa. We used a combined quantitative trait locus (QTL) and candidate gene approach to determine the genetic architecture of sexual isolation in males of two congeneric moths, Heliothis subflexa and Heliothis virescens. We report behavioral and neurophysiological evidence that differential male responses to three female-produced chemicals (Z9-14:Ald, Z9-16:Ald, Z11-16:OAc) that maintain sexual isolation of these species are all controlled by a single QTL containing at least four odorant receptor genes. It is not surprising that pheromone receptor differences could control H. subflexa and H. virescens responses to Z9-16: Ald and Z9-14: Ald, respectively. However, central rather than peripheral level control over the positive and negative responses of H. subflexa and H. virescens to Z11-16: OAc had been expected. Tight linkage of these receptor genes indicates that mutations altering male response to complex blends could be maintained in linkage disequilibrium and could affect the speciation process. Other candidate genes such as those coding for pheromone binding proteins did not map to this QTL, but there was some genetic evidence of a QTL for response to Z11-16: OH associated with a sensory neuron membrane protein gene.