Distribution of a metabotropic glutatnate receptor, mGluR2, in the central nervous system of the rat and mouse: An immunohistochemical study with a monoclonal antibody
Distribution of a metabotropic glutatnate receptor, mGluR2, in the central nervous system of the rat and mouse: An immunohistochemical study with a monoclonal antibody
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DOI：
10.1016/s0168-0102(97)00120-x
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发表时间：
1998-01-01
影响因子：
2.9
通讯作者：
Mizuno, N
中科院分区：
文献类型：
--
作者：
Ohishi, H;Neki, A;Mizuno, N
采用免疫组织化学方法检测了代谢性谷氨酸受体mGluR2在大鼠和小鼠中枢神经系统中的分布，并建立了针对大鼠mGluR2 n端序列（氨基酸残基87-134）的单克隆抗体。大脑皮层Cajal水平细胞、三角隔核和内侧乳状核神经元、小脑皮层高尔基细胞和单极刷状细胞、耳蜗核高尔基样细胞和单极刷状细胞均可见具有mglur2样免疫反应性的神经元细胞体（mGluR2-LI）。神经pil在副嗅球、副嗅束床核、新皮层、扣带皮层、后皮层、隐下和鼻内皮层、CA1和CA3的腔隙层分子、齿状回分子层、杏仁核周围皮层、杏仁核基底外侧核、前联合床核、尾状壳核、伏隔核等部位被强烈免疫染色；丘脑网状核，丘脑腹前核和室旁核，小脑皮层颗粒层，前部和腹侧被盖核，耳蜗核颗粒层，外侧网状核的细胞旁部分。白质和纤维束的许多轴突也被免疫染色。未发现含有mGluR2-LI的胶质细胞。mGluR2-LI在大鼠和小鼠之间的分布模式没有明显的物种差异。结果表明，mGluR2不仅在躯体-树突结构域表达，还在兴奋性和抑制性神经元的轴突结构域表达。(C) 1998爱思唯尔科学爱尔兰有限公司版权所有。
The distribution of a metabotropic glutamate receptor mGluR2 in the central nervous system was immunohistochemically examined in the rat and mouse with a monoclonal antibody raised against an N-terminal sequence of rat mGluR2 (amino acid residues 87-134). Neuronal cell bodies with mGluR2-like immunoreactivity (mGluR2-LI) were clearly shown in the horizontal cells of Cajal in the cerebral cortex, neurons in the triangular septal nucleus and medial mammillary nucleus, Golgi cells and the unipolar brush cells in the cerebellar cortex, and Golgi-like and unipolar brush-like cells in the cochlear nucleus. Neuropil was intensely immunostained in the accessory olfactory bulb, bed nucleus of the accessory olfactory tract, neocortex, cingulate cortex, retrosplenial cortex, subicular and entorhinal cortices, stratum lacunosum-moleculare of CA1 and CA3, molecular layer of the dentate gyrus, periamygdaloid cortex, basolateral amygdaloid nucleus, bed nucleus of the anterior commissure, caudate-putamen, accumbens nucleus; thalamic reticular nucleus, anteroventral and paraventricular thalamic nuclei, granular layer of the cerebellar cortex, anterior and ventral tegmental nuclei, granular layer of the cochlear nucleus, and parvicellular part of the lateral reticular nucleus. Many axons in the white matter and fiber bundles were also immunostained. No glial cells with mGluR2-LI were found. No particular species differences were found in the distribution pattern of mGluR2-LI between the rat and mouse. The results indicate that mGluR2 is expressed not only in somato-dendritic domain, but also in axonal domain of excitatory and inhibitory neurons. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.