In-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the. NCI 60 proteome
In-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the. NCI 60 proteome
复制标题
DOI：
10.1016/j.bbrc.2013.12.070
复制
发表时间：
2014-03-21
影响因子：
3.1
通讯作者：
Kislinger, Thomas
中科院分区：
文献类型：
--
作者：
Sinha, Ankit;Ignatchenko, Vladimir;Kislinger, Thomas
癌细胞与其基质微环境之间的分子通讯是癌症进展的关键因素。除了经典的分泌途径外，最近有人提出，小膜囊泡是细胞间通讯的替代介质。外泌体携带富含效应子的蛋白质组，能够调节受体细胞的各种功能特性。在这项研究中，使用基于质谱的蛋白质组学对从四种上皮性卵巢癌细胞系（OVCAR3、OVCAR433、OVCAR5 和 SKOV3）中分离的外泌体进行了表征。使用由高效外泌体溶解和最新一代蛋白质组仪器组成的优化工作流程，我们展示了改进的检测深度。我们的癌细胞系外泌体蛋白质组与公共数据 (Exocarta) 和最近发布的 NCI 60 蛋白质组的系统比较揭示了与信号传导生物学和生物标志物发现相关的功能类别的丰富。 (C) 2014 Elsevier Inc. 保留所有权利。
Molecular communication between cancer cells and its stromal microenvironment is a key factor for cancer progression. Alongside classic secretory pathways, it has recently been proposed that small membranous vesicles are alternative mediators of intercellular communication. Exosomes carry an effector-rich proteome with the ability to modulate various functional properties of the recipient cell. In this study, exosomes isolated from four epithelial ovarian cancer cell lines (OVCAR3, OVCAR433, OVCAR5 and SKOV3) were characterized using mass spectrometry-based proteomics. Using an optimized workflow consisting of efficient exosome solubilization and the latest generation of proteomic instrumentation, we demonstrate improved detection depth. Systematic comparison of our cancer cell line exosome proteome against public data (Exocarta) and the recently published NCI 60 proteome revealed enrichment of functional categories related to signaling biology and biomarker discovery. (C) 2014 Elsevier Inc. All rights reserved.