Exosomes released from human induced pluripotent stem cells-derived MSCs facilitate cutaneous wound healing by promoting collagen synthesis and angiogenesis.
Exosomes released from human induced pluripotent stem cells-derived MSCs facilitate cutaneous wound healing by promoting collagen synthesis and angiogenesis.
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人诱导多能干细胞衍生的间充质干细胞释放的外泌体通过促进胶原蛋白合成和血管生成来促进皮肤伤口愈合
DOI：
10.1186/s12967-015-0417-0
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发表时间：
2015-02-01
影响因子：
7.4
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Zhang J;Guan J;Niu X;Hu G;Guo S;Li Q;Xie Z;Zhang C;Wang Y
BackgroundHuman induced pluripotent stem cell-derived mesenchymal stem cells (hiPSC-MSCs) have emerged as a promising alternative for stem cell transplantation therapy. Exosomes derived from mesenchymal stem cells (MSC-Exos) can play important roles in repairing injured tissues. However, to date, no reports have demonstrated the use of hiPSC-MSC-Exos in cutaneous wound healing, and little is known regarding their underlying mechanisms in tissue repair.MethodshiPSC-MSC-Exos were injected subcutaneously around wound sites in a rat model and the efficacy of hiPSC-MSC-Exos was assessed by measuring wound closure areas, by histological and immunofluorescence examinations. We also evaluated the in vitro effects of hiPSC-MSC-Exos on both the proliferation and migration of human dermal fibroblasts and human umbilical vein endothelial cells (HUVECs) by cell-counting and scratch assays, respectively. The effects of exosomes on fibroblast collagen and elastin secretion were studied in enzyme-linked immunosorbent assays and quantitative reverse-transcriptase–polymerase chain reaction (qRT-PCR). In vitro capillary network formation was determined in tube-formation assays.ResultsTransplanting hiPSC-MSC-Exos to wound sites resulted in accelerated re-epithelialization, reduced scar widths, and the promotion of collagen maturity. Moreover, hiPSC-MSC-Exos not only promoted the generation of newly formed vessels, but also accelerated their maturation in wound sites. We found that hiPSC-MSC-Exos stimulated the proliferation and migration of human dermal fibroblasts and HUVECs in a dose-dependent manner in vitro. Similarly, Type I, III collagen and elastin secretion and mRNA expression by fibroblasts and tube formation by HUVECs were also increased with increasing hiPSC-MSC-Exos concentrations.ConclusionsOur findings suggest that hiPSC-MSC-Exos can facilitate cutaneous wound healing by promoting collagen synthesis and angiogenesis. These data provide the first evidence for the potential of hiPSC-MSC-Exos in treating cutaneous wounds.
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影响因子：
5
作者：
Lai, Ruenn Chai;Arslan, Fatih;Lim, Sai Kiang
通讯作者：
Lim, Sai Kiang
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影响因子：
4.8
作者：
Cai, Jinglei;Li, Wen;Pei, Duanqing
通讯作者：
Pei, Duanqing
影响因子：
15.9
作者：
Roccaro, Aldo M.;Sacco, Antonio;Ghobrial, Irene M.
通讯作者：
Ghobrial, Irene M.
人骨髓间充质干细胞来源的细胞外囊泡促进大鼠心肌梗死模型中的血管生成
影响因子：
4.7
作者：
Bian, Suyan;Zhang, Liping;Yu, Hepeng
通讯作者：
Yu, Hepeng
影响因子：
64.8
作者：
Park, In-Hyun;Zhao, Rui;Daley, George Q.
通讯作者：
Daley, George Q.