Effects of high potassium depolarization, carbachol and oxytocin on the Lanthanum-resistant 45Ca fraction in rat myometrium.
Effects of high potassium depolarization, carbachol and oxytocin on the Lanthanum-resistant 45Ca fraction in rat myometrium.
复制标题
高钾去极化、卡巴胆碱和催产素对大鼠子宫肌层耐镧 45Ca 部分的影响。
DOI：
10.1254/jjp.33.1
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
K. Kubota
中科院分区：
文献类型：
--
作者：
T. Uruno;T. Sekiguchi;N. Sunagane;Y. Matsuoka;K. Kubota
用改良的镧法比较了高钾、氨甲酰胆碱和催产素对雌二醇处理的大鼠和去卵巢大鼠子宫纵肌~（40）Ca-~（45）Ca交换的影响。“用60 mM K+处理雌激素化或卵巢切除的子宫肌层，在5分钟、10分钟、30分钟和60分钟的孵育时间内显著增加了45 Ca空间。高K+对雌激素化子宫肌层~（45）Ca的升高作用明显大于去卵巢子宫肌层。卡巴胆碱（2 × 10（-6）M）在所有孵育期对~（45）Ca间隙的影响很小，但10分钟时除外。高浓度卡巴胆碱（2 × 10（-4）M）在雌激素化的子宫肌层中孵育10分钟后，45 Ca空间显著增加，而在卵巢切除的子宫中，在孵育10分钟和30分钟时，观察到45 Ca空间略有增加。2 × 10（-4）M卡巴胆碱对雌激素化子宫肌层~（45）Ca空间的增加大于去卵巢子宫肌层。催产素（10（-4）和10（-3）单位/ml）在雌激素化子宫中孵育10分钟时和孵育10分钟后增加~（45）Ca空间，而在去卵巢子宫中，催产素（10（-3）和10（-2）单位/ml）对~（45）Ca空间的增加几乎没有影响。罂粟碱可抑制卡巴胆碱和催产素引起的~（45）Ca空间的增加。这些结果表明，在依赖于Ca ~（2+）在介质中可能存在的差异，在高K ~+，氨甲酰胆碱和催产素诱导的子宫平滑肌收缩，在雌二醇处理的子宫肌层的收缩可能更依赖于细胞外Ca ~（2+）比那些在卵巢切除的。
The effects of high potassium, carbachol and oxytocin on 40Ca-45Ca exchange in the estradiol-treated rat uterine longitudinal muscle were compared with those in the ovariectomized one using the modified "Lanthanum Method." The treatment of estrogenized or ovariectomized myometrium with 60 mM K+ significantly increased the 45Ca space at 5-, 10-, 30- and 60-min incubation times. The increase in the 45Ca by high-K+ in the estrogenized myometrium was much greater than that in the ovariectomized one. Carbachol (2 X 10(-6) M) had little influence on the 45Ca space at all incubation periods with the exception of the 10-min one in both uteri. The higher concentration of carbachol (2 X 10(-4) M) in the estrogenized myometrium produced a marked increase in the 45Ca space after the 10-min incubation, while in the ovariectomized uterus, a slight increase in the 45Ca space was observed at the 10- and 30-min incubation times. The increase in the 45Ca space by 2 X 10(-4) M carbachol in the estrogenized myometrium was greater than that in the ovariectomized one. Oxytocin (10(-4) and 10(-3) units/ml) increased the 45Ca space at and after the 10-min incubation times in the estrogenized uterus, while in the ovariectomized uterus, oxytocin (10(-3) and 10(-2) units/ml) showed little influence on the increase in the 45Ca space. The increase in 45Ca space by carbachol and oxytocin was inhibited by papaverine. These results suggest that differences in dependence upon Ca2+ in the medium may exist in contractions induced by high-K+, carbachol and oxytocin in the uterine smooth muscle and that the contractions in the estradiol-treated myometrium may be more dependent upon extracellular Ca2+ than those in the ovariectomized one.