Chemical transformations of succinic acid recovered from fermentation broths by a novel direct vacuum distillation-crystallisation method
Chemical transformations of succinic acid recovered from fermentation broths by a novel direct vacuum distillation-crystallisation method
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DOI：
10.1039/b813409j
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发表时间：
2009-01-01
期刊：
影响因子：
9.8
通讯作者：
Clark, James H.
中科院分区：
文献类型：
--
作者：
Luque, Rafael;Lin, Carol S. K.;Clark, James H.
报道了一种替代传统钙沉淀的新方法(直接结晶法)，以从已定义的小麦发酵液中获得琥珀酸(SA)。用该方法成功地从发酵液中回收了SA晶体。与略微改进的传统钙沉淀法(分别为90%和24%)相比，直接结晶法获得了更高的SA结晶纯度(95%)和产率(70%)。然后使用一系列固体酸，包括我们的酸性可调介孔碳质材料Starbon(R)酸，研究了两种回收的SA晶体向高附加值衍生物的化学转化(例如酯化)。结果表明，无论在何种反应条件下，SA晶体都能以较高的产率和选择性成功地转化为单酯和双酯。反应活性顺序为：纯SA晶体>；SA晶体和确定的FB>；结果表明，从实际发酵液中可以有效地提纯SA，这表明了将发酵和下游加工相结合对优化SA的发酵生产及其化学转化以生产高附加值衍生物的重要性。
A novel alternative methodology ( direct crystallisation) to the traditional calcium precipitation to obtain succinic acid ( SA) from defined and wheat-based fermentation broths is reported. SA crystals were successfully recovered from fermentation broths (FB) using this method. A higher SA crystal purity (95%) and yield (70%) were obtained in the direct crystallisation method compared to a slightly modified traditional calcium precipitation method (90% and 24%, respectively). Chemical transformations (e. g. esterifications) to high-added value derivatives of both recovered SA crystals were then investigated using a range of solid acids including our acidic tunable mesoporous carbonaceous materials denoted as Starbon (R) acids. Results showed that SA crystals could be successfully converted into mono- and diesters in high yields and selectivities employing solid acids regardless of the reaction conditions. The order of reactivity was found to be: pure SA crystals > SA crystals from defined FB > FB SA crystals. Results demonstrate that SA can be effectively purified from actual fermentation broths, showing the importance of integrating the fermentation and downstream processing to optimise the fermentative production of SA and its chemical transformations to produce high-added value derivatives.