INVIVO CALCIUM ELEVATIONS IN THYMOCYTES WITH T-CELL RECEPTORS THAT ARE SPECIFIC FOR SELF LIGANDS
INVIVO CALCIUM ELEVATIONS IN THYMOCYTES WITH T-CELL RECEPTORS THAT ARE SPECIFIC FOR SELF LIGANDS
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DOI：
10.1126/science.1621102
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发表时间：
1992-07-03
期刊：
影响因子：
56.9
通讯作者：
JUNE, CH
中科院分区：
文献类型：
--
作者：
NAKAYAMA, T;UEDA, Y;JUNE, CH
胸腺中 T 细胞受体 (TCR) 库的选择可能涉及在与带有自身配体的胸腺基质细胞相互作用后，在发育中的胸腺细胞中转导的 TCR 介导的信号。 TCR 转导信号应具有可识别的结果，以区分 TCR 已被自身配体参与的胸腺细胞与 TCR 未参与的胸腺细胞。在表达具有明确特异性的转基因TCR的胸腺细胞中，大量细胞内钙浓度升高，但仅当驻留在表达其自身配体的负选择胸腺中时。因此，发育中的胸腺细胞受到体内内源配体的刺激以动员细胞内钙，并且细胞内钙浓度的增加可能反映了与胸腺负选择相关的胸腺内信号传导的后果。
Selection of the T cell receptor (TCR) repertoire in the thymus probably involves TCR-mediated signals transduced in developing thymocytes after interaction with thymic stromal cells bearing self ligands. TCR-transduced signals should have identifiable consequences that would distinguish thymocytes whose TCRs have been engaged by self ligands from those whose TCRs have not. Among thymocytes expressing a transgenic TCR of defined specificity, a large number had elevated intracellular calcium concentrations but only when resident in a negatively selecting thymus in which their self ligand was expressed. Thus, developing thymocytes are stimulated by endogenous ligands in vivo to mobilize intracellular calcium, and increased intracellular calcium concentrations may reflect the consequences of intrathymic signaling associated with thymic negative selection.