The WOR1 5' untranslated region regulates white-opaque switching in Candida albicans by reducing translational efficiency.
The WOR1 5' untranslated region regulates white-opaque switching in Candida albicans by reducing translational efficiency.
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DOI：
10.1111/mmi.13014
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发表时间：
2015-07
影响因子：
3.6
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Guan Z;Liu H
人类真菌病原体C和白色念珠菌经历了白色-不透明表型转换，这增强了它对宿主生态位的适应。切换是由互锁反馈回路的转录调控网络控制的，该网络作用于WOR 1的转录，WOR 1是白色不透明切换的主要调控因子，但该网络在翻译水平上的调控尚未被探索。在这里，我们发现WOR 1的长5 '非翻译区调节白色-不透明表型。wor15 ' UTR的缺失促进了白光到不透明的转换，稳定了不透明状态。wor15 ' UTR降低了翻译效率和转录本与多体的关联。在长5 ' UTR的细胞命运和形态的其他关键调节因子中也观察到多聚体关联减少。总的来说，我们发现了一个在翻译水平上的白色-不透明转换的新调控步骤。这种翻译调控与白色念珠菌细胞命运和形态的许多关键调控有关。
The human fungal pathogen C andida albicans undergoes white‐opaque phenotypic switching, which enhances its adaptation to host niches. Switching is controlled by a transcriptional regulatory network of interlocking feedback loops acting on the transcription of WOR 1, the master regulator of white‐opaque switching, but regulation of the network on the translational level is not yet explored. Here, we show that the long 5′ untranslated region of WOR 1 regulates the white‐opaque phenotype. Deletion of the WOR 1 5′ UTR promotes white‐to‐opaque switching and stabilizes the opaque state. The WOR 1 5′ UTR reduces translational efficiency and the association of the transcript with polysomes. Reduced polysome association was observed for additional key regulators of cell fate and morphology with long 5′ UTR as well. Overall, we find a novel regulatory step of white‐opaque switching at the translational level. This translational regulation is implicated for many key regulators of cell fate and morphology in C . albicans.