CA2+/CALMODULIN PROTEIN-KINASE AND PROTEIN-KINASE-C EXPRESSION DURING DEVELOPMENT OF RAT HIPPOCAMPUS
CA2+/CALMODULIN PROTEIN-KINASE AND PROTEIN-KINASE-C EXPRESSION DURING DEVELOPMENT OF RAT HIPPOCAMPUS
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DOI：
10.1159/000111365
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发表时间：
1993-11-01
影响因子：
2.9
通讯作者：
SCHLINGENSIEPEN, KH
中科院分区：
文献类型：
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作者：
HERMS, J;ZURMOHLE, U;SCHLINGENSIEPEN, KH
采用原位杂交组织化学方法检测了ii型钙/钙调素依赖性蛋白激酶(CAM-KII α) α亚基基因和蛋白激酶C (PKC γ) γ亚种基因在出生后大鼠大脑发育过程中的表达。CAM-KII α在不同海马亚区依次表达，从出生时在CA3锥体细胞中表达开始，随后在出生后（PND） 5在齿状回外叶和CA1中表达，最后在齿状回内叶PND 8中表达。相比之下，海马CA1和CA3亚区PKC γ的表达同时上升，齿状回的表达很少。这种表达方式与CA1和CA3中锥体细胞相似的成熟状态相对应，而在大鼠海马发育过程中，CAM-KII α的表达遵循传入层压的时间表，与CA3相比，CA1中的传入层压延迟。此外，我们发现CAM-KII α在出生后第二周结束时在海马亚区CA1和CA3中短暂过表达，这与n -甲基- d -天冬氨酸受体结合的发展一致。
The expression of the genes encoding the alpha subunit of type-II calcium/calmodulin-dependent protein kinase (CAM-KII alpha) and the gamma subspecies of protein kinase C (PKC gamma) was examined throughout postnatal rat brain development by in situ hybridization histochemistry. CAM-KII alpha was found to be expressed sequentially over the different hippocampal subregions, beginning with expression in the pyramidal cells of CA3 at birth, followed by expression in the external blade of the dentate gyrus and in CA1 on postnatal day (PND) 5 and, finally, on PND 8 in the internal blade of the dentate gyrus. PKC gamma expression, in contrast, rose simultaneously in the hippocampal subregions CA1 and CA3, with little expression over the dentate gyrus. This fashion of expression corresponds to the similar maturational state of the pyramidal cells in CA1 and CA3, whereas CAM-KII alpha expression during development of the rat hippocampus follows the time table of afferent lamination, which is delayed in CA1 compared to CA3. Furthermore, we found a temporal overexpression of CAM-KII alpha in the hippocampal subfields CA1 and CA3 at the end of the second postnatal week which coincides with the development of N-methyl-D-aspartate receptor binding.