Metabolomics and correlation network analysis of follicular fluid reveals associations between L-tryptophan, L-tyrosine and polycystic ovary syndrome
Metabolomics and correlation network analysis of follicular fluid reveals associations between L-tryptophan, L-tyrosine and polycystic ovary syndrome
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卵泡液的代谢组学和相关网络分析揭示了 L-色氨酸、L-酪氨酸和多囊卵巢综合征之间的关联
DOI：
10.1002/bmc.4993
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发表时间：
2020-11-05
影响因子：
1.8
通讯作者：
Qiao, Jie
中科院分区：
文献类型：
--
作者：
Hou, Entai;Zhao, Yue;Qiao, Jie
多囊卵巢综合征（PCOS）是生殖年龄女性的内分泌和代谢疾病。一些研究研究了PCOS患者血浆和滤泡液的代谢改变，但他们没有控制肥胖或胰岛素抵抗（IR）。另外，代谢物的相关分析很少。因此，在这项研究中，我们旨在检查由于PCOS的发病机理，确定轮毂代谢物并研究其与雄激素的关联。我们应用了GC-MS平台，再加上相关网络方法，以分析来自32名无肥胖症和IR和31名健康女性的32名PCOS患者的卵泡液样品。 PCOS患者中的三十个显着改变的代谢物在氨基酸代谢中富集。在代谢相关网络中，将L-苯基丙氨酸，L-色氨酸，焦谷氨酸，L-酪氨酸，L-柠檬氨酸和L谷氨酸作为轮毂代谢物筛选为轮毂代谢产物。其中，升高的L-色氨酸和L-酪氨酸的增加是轮毂代谢物，它们对PCOS的代谢变化产生了更大的影响。此外，L-色氨酸和L-酪氨酸与PCOS中的血清雄激素水平显着相关。我们的结果表明，氨基酸代谢的疾病，尤其是色氨酸和酪氨酸代谢，可能在没有肥胖症和IR的诱发女性的PCOS发展中起重要作用。
Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disorder in women of reproductive age. Some studies have investigated metabolic alterations in plasma and follicular fluid from PCOS patients, but they did not control for obesity or insulin resistance (IR); additionally, correlation analysis of metabolites is sparse. Accordingly, in this study, we aimed to examine metabolic differences owing to the pathogenesis of PCOS, identify the hub metabolites and investigate its associations with androgens. We applied GC-MS platform coupled with a correlation network approach to analyze follicular fluid samples from 32 PCOS patients without obesity and IR and 31 healthy women. Thirty significantly altered metabolites in PCOS patients were enriched in amino acid metabolism. l-Phenylalanine, l-tryptophan, pyroglutamic acid, l-tyrosine, l-leucine and l-valine were screened as hub metabolites in metabolic correlation network. Among them, increased l-tryptophan and l-tyrosine were altered hub metabolites, and they had a more significant impact on the metabolic change of PCOS. In addition, l-tryptophan and l-tyrosine were significantly positively associated with serum androgens levels in the PCOS. Our results suggest that disorders of amino acid metabolism, especially tryptophan and tyrosine metabolism, might play an important role in the development of PCOS in predisposed women without obesity and IR.