cGMP-dependent protein kinase activates Ca-activated K channels in cerebral artery smooth muscle cells.
cGMP-dependent protein kinase activates Ca-activated K channels in cerebral artery smooth muscle cells.
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DOI：
10.1152/ajpcell.1993.265.1.c299
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发表时间：
1993-07
期刊：
影响因子：
--
通讯作者：
B. E. Robertson;R. Schubert;Jorgen Hescheler;M. Nelson
中科院分区：
文献类型：
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作者：
B. E. Robertson;R. Schubert;Jorgen Hescheler;M. Nelson
鸟苷-3′，5′-环单磷酸（cGMP）依赖性蛋白激酶（cGMP- pk）在介导血管扩张剂对一氧化氮（NO）和其他硝基血管扩张剂的反应中起核心作用。目前尚不清楚cGMP-PK是否影响平滑肌中的钙活化钾通道（KCa通道）或任何其他类型的离子通道。我们在这里提供了第一个直接证据，证明cGMP-PK可以激活动脉平滑肌细胞中的KCa通道。我们证明NO和cGMP的膜渗透性类似物可以激活细胞上斑块中的KCa通道大约两倍。此外，cGMP- pk，在ATP和cGMP直接添加到细胞内表面的内向外斑块，增加通道活性约8倍。这些结果表明cgmp - pk介导的KCa通道激活可能有助于NO和其他硝基血管扩张剂的作用。
Guanosine-3',5'-cyclic monophosphate (cGMP)-dependent protein kinase (cGMP-PK) plays a central role in the mediation of the vasodilator response to nitric oxide (NO) and other nitrovasodilators. It is unclear whether cGMP-PK affects calcium-activated potassium channels (KCa channels) or any other type of ion channel in smooth muscle. We provide here the first direct evidence that cGMP-PK can activate KCa channels in arterial smooth muscle cells. We demonstrate that NO and a membrane-permeable analogue of cGMP can activate KCa channels in on-cell patches approximately twofold. Furthermore, cGMP-PK, in the presence of ATP and cGMP added directly to the intracellular surface of inside-out patches, increases channel activity by approximately eightfold. These results suggest that cGMP-PK-mediated activation of KCa channels may contribute to the actions of NO and other nitrovasodilators.