ASSIGNMENT OF THE GENOME SEGMENTS OF BLUETONGUE VIRUS TYPE-1 TO THE PROTEINS WHICH THEY ENCODE
ASSIGNMENT OF THE GENOME SEGMENTS OF BLUETONGUE VIRUS TYPE-1 TO THE PROTEINS WHICH THEY ENCODE
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DOI：
10.1016/0042-6822(84)90131-4
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发表时间：
1984-01-01
期刊：
影响因子：
3.7
通讯作者：
SANGAR, DV
中科院分区：
文献类型：
--
作者：
MERTENS, PPC;BROWN, F;SANGAR, DV
通过聚丙烯酰胺凝胶电泳将纯化的蓝舌病毒1型（BTV 1）基因组ds[双链]RNA分为10个大小类别。这些基因组片段从凝胶中单独回收，用氢氧化甲基汞变性，并在兔网织红细胞裂解物系统中进行体外翻译。通过聚丙烯酰胺凝胶电泳将体外合成的病毒特异性蛋白质与纯化的病毒颗粒和核心的蛋白质以及受感染的[仓鼠肾] BHK 21 细胞（体内）中合成的病毒特异性蛋白质进行比较。通过对其部分蛋白酶消化物进行电泳分析，证实了体内和体外合成的那些表现出相似电泳迁移率的 BTV 1 蛋白的身份。这些实验的结果允许将 BTV 1 的每个基因组片段分配给它编码的蛋白质，从而分配给受感染细胞中发现的结构和非结构蛋白质。
The purified genomic ds[double-stranded]RNA of bluetongue virus type 1 (BTV 1) was separated into 10 size classes by polyacrylamide gel electrophoresis. These genome segments were recovered individually from the gel, denatured with methylmercuric hydroxide and translated in vitro in a rabbit reticulocyte lysate system. The virus-specific proteins synthesized in vitro were compared by polyacrylamide gel electrophoresis with proteins from purified virus particles and cores, and with virus-specified proteins synthesized in infected [hamster kidney] BHK 21 cells (in vivo). The identity of those BTV 1 proteins synthesized in vivo and in vitro which showed similar electrophoretic mobilities, was confirmed by electrophoretic analysis of their partial protease digests. The results of these experiments have allowed the assignment of each of the genome segments of BTV 1 to the protein(s) which it encodes, and consequently to the structural and nonstructural proteins found in the infected cell.