GALANIN-LIKE IMMUNOREACTIVITY IN CHOLINERGIC NEURONS OF THE SEPTUM-BASAL FOREBRAIN COMPLEX PROJECTING TO THE HIPPOCAMPUS OF THE RAT
GALANIN-LIKE IMMUNOREACTIVITY IN CHOLINERGIC NEURONS OF THE SEPTUM-BASAL FOREBRAIN COMPLEX PROJECTING TO THE HIPPOCAMPUS OF THE RAT
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DOI：
10.1016/0006-8993(85)91228-4
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发表时间：
1985-12-23
期刊：
影响因子：
2.9
通讯作者：
WAINER, BH
中科院分区：
文献类型：
--
作者：
MELANDER, T;STAINES, WA;WAINER, BH
现在已经认识到，基底前脑中有几组胆碱能神经元，它们直接投射到不同的皮质区域。神经肽甘丙肽（GAL）的分布的免疫组织化学研究表明，这些脑区，内侧隔和斜角带，含有大量的GAL免疫反应神经元。在本研究中，双染色技术使用的抗体对胆碱乙酰转移酶（ChAT）显示，GAL和ChAT样免疫反应共定位在内侧隔和斜角带内的胆碱能神经元的亚群。GAL和ChAT免疫反应的共定位没有看到发生在其他组的前脑胆碱能神经元。免疫组织化学进行注射后的荧光逆行示踪剂到海马结构显示，两个ChAT GAL和ChAT含神经元项目的海马结构。GAL的问题作为一个调制器的mamminergic传输在这个投影进行了讨论。
It is now well recognized that there are several groups of cholinergic neurons in the basal forebrain with direct projections to various cortical regions. Immunohistochemical investigations of the distribution of the neuropeptide galanin (GAL) have shown that two of these brain areas, the medial septum and diagonal band, contained large numbers of GAL-immunoreactive neurons. In the present study, double staining techniques using antibodies raised against choline acetyltransferase (ChAT) revealed that GAL- and ChAT-like immunoreactivities are colocalized within a subpopulation of the cholinergic neurons within the medial septum and diagonal band. This colocalization of GAL- and ChAT-immunoreactivities was not seen to occur within other groups of forebrain cholinergic neurons. Immunohistochemistry carried out subsequent to injections of fluorescent retrograde tracers into the hippocampal formation revealed that both ChAT GAL- and ChAT-containing neurons project to the hippocampal formation. The question of GAL as a modulator of chloinergic transmission in this projection is discussed.