Japanese encephalitis virus interacts with vimentin to facilitate its entry into porcine kidney cell line
Japanese encephalitis virus interacts with vimentin to facilitate its entry into porcine kidney cell line
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DOI：
10.1016/j.virusres.2011.06.001
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发表时间：
2011-09-01
期刊：
影响因子：
5
通讯作者：
Desai, Anita
中科院分区：
文献类型：
--
作者：
Das, Soma;Ravi, Vasanthapuram;Desai, Anita
日本脑炎病毒（JEV）需要宿主细胞表面存在一种无法解释的细胞受体才能进入细胞。JEV包膜（E）蛋白已被证明在与细胞的附着中起重要作用。通过使用广泛接受的技术，病毒覆盖蛋白结合试验（VOPBA），一种约60 kDa的蛋白质分子，通过基质辅助激光解吸电离飞行时间质谱（MALDI TOF）鉴定为波形蛋白，被认为是猪肾（PS）细胞上的JEV的可能受体。此外，抗波形蛋白单克隆抗体能够阻断JEV进入PS细胞。此外，免疫共沉淀测定证实了PS细胞上存在的波形蛋白与JEV-E蛋白相互作用。这些观察结果表明，波形蛋白作为一个假定的受体JEV在猪肾细胞。(C)2011 Elsevier B. V.保留所有权利。
Japanese encephalitis virus (JEV) requires the presence of an inexplicable cellular receptor on the surface of the host cell for its entry into the cell. The JEV envelope (E) protein has been shown to play an important role in attachment to cells. By using a widely accepted technique, virus overlay protein binding assay (VOPBA), a protein molecule of approximately 60 kDa, identified as vimentin by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI TOF), was recognized on porcine kidney (PS) cells as a possible receptor for JEV. Further, anti-vimentin monoclonal antibodies were able to block JEV entry into the PS cells. Additionally, co-immunoprecipitation assay confirmed that vimentin protein present on the PS cells interacts with the JEV-E protein. These observations indicate that vimentin serves as a putative receptor for JEV in porcine kidney cells. (C) 2011 Elsevier B.V. All rights reserved.