Fate of the initial follicle pool: Empirical and mathematical evidence supporting its sufficiency for adult fertility
Fate of the initial follicle pool: Empirical and mathematical evidence supporting its sufficiency for adult fertility
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DOI：
10.1016/j.ydbio.2006.06.023
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发表时间：
2006-10-01
影响因子：
2.7
通讯作者：
Woodruff, Teresa K.
中科院分区：
文献类型：
--
作者：
Bristol-Gould, Sarah K.;Kreeger, Pamela K.;Woodruff, Teresa K.
最初卵泡池在雌性成年哺乳动物生育力中的重要性最近一直存在争议。利用一个数学模型的动态卵泡进展（原始初级到次级），我们研究了初始卵泡池是否足够的成年生育能力，通过生殖衰老的CD 1小鼠。从出生后第6天到第12个月，对每个阶段的卵泡进行计数，并将数据拟合到一系列一阶微分方程，这些一阶微分方程代表两种机制：原始卵泡的初始池作为唯一的卵泡来源（固定池模型），或原始卵泡池补充生殖系干细胞（干细胞模型）。固定池模型拟合实验数据，准确地代表了4-6月龄时达到的最大观察到的初级卵泡数。尽管通过SSEA-1免疫染色不能鉴定生殖系干细胞，但使用一系列从头原始卵泡产生率来测试干细胞模型。干细胞模型未能描述观察到的卵泡随时间的减少，并且在小鼠的早期可育寿命期间初级卵泡的积累和随后的减少不平行。我们的研究结果同意既定的教条，即卵巢卵泡的初始禀赋并没有得到可观数量的干细胞的补充;相反，它足以确保成年小鼠的生育需求。(c)2006年爱思唯尔公司All rights reserved.
The importance of the initial follicle pool in fertility in female adult mammals has recently been debated. Utilizing a mathematical model of the dynamics of follicle progression (primordial to primary to secondary), we examined whether the initial follicle pool is sufficient for adult fertility through reproductive senescence in CD 1 mice. Follicles in each stage were counted from postnatal day 6 through 12 months and data were fit to a series of first-order differential equations representing two mechanisms: an initial pool of primordial follicles as the only follicle source (fixed pool model), or an initial primordial follicle pool supplemented by germline stem cells (stem cell model). The fixed pool model fit the experimental data, accurately representing the maximum observed primary follicle number reached by 4-6 months of age. Although no germline stem cells could be identified by SSEA-1 immunostaining, the stem cell model was tested using a range of de novo primordial follicle production rates. The stem cell model failed to describe the observed decreases in follicles over time and did not parallel the accumulation and subsequent reduction in primary follicles during the early fertile lifespan of the mouse. Our results agree with established dogma that the initial endowment of ovarian follicles is not supplemented by an appreciable number of stem cells; rather, it is sufficient to ensure the fertility needs of the adult mouse. (c) 2006 Elsevier Inc. All rights reserved.