Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation
Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation
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DOI：
10.1038/nm879
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发表时间：
2003-06-01
期刊：
影响因子：
82.9
通讯作者：
Jain, RK
中科院分区：
文献类型：
--
作者：
Brown, E;McKee, T;Jain, RK
胶原蛋白的含量和结构在控制治疗分子在肿瘤中的递送中是必不可少的。因此，对肿瘤组织活检的胶原蛋白进行简单的组织学染色可用于评估肿瘤中分子治疗的可及性。在这里，我们表明，它是可能的光学图像纤维状胶原蛋白在肿瘤生长的小鼠使用二次谐波产生（SHG）。使用这种非侵入性技术，我们估计相对扩散障碍，量化的胶原蛋白修饰的动力学药物干预后，并提供了机制的见解，以改善激素松弛素诱导的扩散运输。这项技术可以为基础科学家和临床医生提供增强的能力，以估计分子疗法的相对渗透性。
The content and structure of collagen is essential in governing the delivery of therapeutic molecules in tumors. Thus, simple histological staining of tumor tissue biopsies for collagen could be used to assess the accessibility of molecular therapeutics in tumors. Here we show that it is possible to optically image fibrillar collagen in tumors growing in mice using second-harmonic generation (SHG). Using this noninvasive technique, we estimated relative diffusive hindrance, quantified the dynamics of collagen modification after pharmacologic intervention and provided mechanistic insight into improved diffusive transport induced by the hormone relaxin. This technology could offer basic scientists and clinicians an enhanced ability to estimate the relative penetrabilities of molecular therapeutics.