The distribution of angiotensinogen in dog brain studied by cell fractionation.
The distribution of angiotensinogen in dog brain studied by cell fractionation.
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通过细胞分级研究血管紧张素原在狗脑中的分布。
DOI：
10.1210/endo-103-2-492
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发表时间：
1978
期刊：
影响因子：
4.8
通讯作者：
I. Reid
中科院分区：
文献类型：
--
作者：
B. Morris;I. Reid
脑脊液中含有血管紧张素原，但其来源和作用尚不清楚。脑细胞中含有血管紧张素原颗粒的可能性进行了检查细胞分级。狗中脑和前脑的亚细胞组分在37 ℃、pH7.4下与肾素孵育60分钟后产生血管紧张素I。中脑和前脑的结果几乎相同。血管紧张素原的最高比浓度是在可溶性部分。一些血管紧张素原存在于颗粒组分中，但等密度梯度离心和使用125 I标记的人血清白蛋白和125 I标记的犬血管紧张素原制剂的对照实验表明，这可以完全由可溶性污染物和颗粒（可能是溶酶体）在分级过程中的非特异性摄取来解释。全组织中的浓度约为0.001 μ g/ml。45 pmol/g组织和基于脑脊液浓度的计算表明，所有...
Cerebrospinal fluid contains angiotensinogen; however, its origin and role are not known. The possibility that brain cells contain angiotensinogen in granules was examined by cell fractionation. Subcellular fractions of midbrain and forebrain of dogs generated angiotensin I over a period of 60 min when incubated with renin at 37 C, pH 7.4. The results for midbrain and forebrain were virtually identical. The highest specific concentration of angiotensinogen was in the soluble fraction. Some angiotensinogen was present in granule fractions, but it was shown by isopycnic gradient centrifugation and in control experiments using 125I-labeled human serum albumin and an 125I-labeled preparation of dog angiotensinogen that this could be accounted for entirely by soluble contaminant and nonspecific uptake by granules, probably lysosomes, during the fractionation procedure. The concentration in whole tissue was ca. 45 pmol/g tissue and calculations based on the concentration in cerebrospinal fluid suggest that all ...