MOVEMENT OF MYOSIN-COATED FLUORESCENT BEADS ON ACTIN CABLES INVITRO
MOVEMENT OF MYOSIN-COATED FLUORESCENT BEADS ON ACTIN CABLES INVITRO
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DOI：
10.1038/303031a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
SPUDICH, JA
中科院分区：
文献类型：
--
作者：
SHEETZ, MP;SPUDICH, JA
肌球蛋白包被的荧光珠沿着有组织的肌动蛋白丝阵列单向移动，在丽藻中，其平均速度与肌肉和其他细胞中的体内运动速率相似。肌球蛋白产生动力，因为运动是ATP依赖性的，并且被肌球蛋白头的失活所阻断。在没有双极粗丝的情况下发生的运动提供了肌球蛋白运动的定量测定。
Myosin-coated fluorescent beads move unidirectionally along organized actin filament arrays in the alga, Nitella, with an average velocity similar to in vivo rates of movement in muscle and other cells. The myosin produces the motive force, as the movement is ATP-dependent and is blocked by inactivation of the myosin heads. The movement, which occurs in the absence of bipolar thick filaments, provides a quantitative assay for myosin motility.