Interaction of the cAMP receptor protein with the lac promoter.
Interaction of the cAMP receptor protein with the lac promoter.
复制标题
cAMP 受体蛋白与 lac 启动子的相互作用。
DOI：
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发表时间：
1980
影响因子：
14.9
通讯作者：
R. B. Simpson
中科院分区：
文献类型：
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作者：
R. B. Simpson
环AMP受体蛋白（CRP）通过结合lac启动子刺激乳糖操纵子的转录。我已经确定了启动子中胸腺嘧啶的5个位置它们靠近结合的CRP。虽然用取代胸腺嘧啶的5-溴嘧啶对DNA进行紫外线照射通常会在溴嘧啶处切割DNA，但与DNA结合的蛋白质会扰乱靠近溴的蛋白质位置的切割。从光化学探针推断的接触和外切酶III对该复合体的溶核攻击结果支持一个模型，即环AMP受体蛋白与启动子结合，与双螺旋的一面对称接触，然后通过与RNA聚合酶接触刺激转录。
The cyclic AMP receptor protein (CRP) stimulates transcription of the lactose operon by binding to the lac promoter. I have identified those 5-positions of thymines in the promoter that lie close to bound CRP. Although ultraviolet irradiation of DNA with 5-bromouracil substituted in place of thymine normally cleaves the DNA at the bromouracils, a protein bound to the DNA can perturb these cleavages at those locations at which the protein lies close to the bromine. The contacts inferred from this photochemical probe and the results of nucleolytic attack of this complex by exonuclease III support a model where the cyclic AMP receptor protein binds to the promoter making symmetrical contacts with one face of the double helix and then stimulates transcription through contacts with RNA polymerase.