Aging-specific expression of Drosophila hsp22
Aging-specific expression of Drosophila hsp22
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DOI：
10.1006/dbio.1998.9147
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发表时间：
1999-03-01
影响因子：
2.7
通讯作者：
Tower, J
中科院分区：
文献类型：
--
作者：
King, V;Tower, J
热休克蛋白22是果蝇在发育和热应激过程中表达量最低的褶状休克基因之一。与此相反，hsp 22被发现是最丰富的表达hs基因在果蝇老化。在衰老过程中，hsp 22 RNA在头部被诱导60倍，在腹部和胸部的诱导水平略低。其他hs基因RNA的诱导小于或等于3倍，除了hsp 23，其在胸部中的诱导类似于5倍。使用大鼠抗-hsp 22多克隆抗血清检测hsp 22蛋白，其被诱导>150倍，在眼组织中具有特别丰富的表达。在转基因果蝇中，通过hsp 22：lacZ融合报告基因构建体再现hsp 22的衰老特异性诱导。转基因果蝇中特异性启动子突变的分析表明，衰老过程中热休克蛋白22的诱导需要功能性热休克反应元件。最后，hsp 22 RNA和蛋白表达模式的比较表明，衰老特异性表达的hsp 22的调节在转录和转录后水平。hsp 22的衰老特异性诱导与当前的衰老进化理论有关，（C）1999年学术出版社。
hsp22 is among the least abundantly expressed Drosophila Pleat shock (hs) genes during both development and heat stress. In contrast, hsp22 was found to be the most abundantly expressed hs gene during Drosophila aging. During aging, hsp22 RNA was induced 60-fold in the head, with somewhat lower level induction in abdomen and thorax. Induction of the other hs gene RNAs was less than or equal to 3-fold, except for hsp23, which was induced similar to 5-fold in thorax. hsp22 protein was detected using rat anti-hsp22 polyclonal antisera and was induced >150-fold, with particularly abundant expression in eye tissue. Aging-specific induction of hsp22 was reproduced by hsp22:lacZ fusion reporter constructs in transgenic flies. Analysis of specific promoter mutations in transgenic flies indicated that functional heat shock response elements are required for hsp22 induction during aging. Finally, comparison of hsp22 RNA and protein expression patterns suggests that aging-specific expression of hsp22 is regulated at both the transcriptional and the posttranscriptional levels. Aging-specific induction of hsp22 is discussed with regard to current evolutionary theories of aging, (C) 1999 Academic Press.