PAR3β, a novel homologue of the cell polarity protein PAR3, localizes to tight junctions
PAR3β, a novel homologue of the cell polarity protein PAR3, localizes to tight junctions
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DOI：
10.1016/s0006-291x(02)02698-0
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发表时间：
2002-12-13
影响因子：
3.1
通讯作者：
Sumimoto, H
中科院分区：
文献类型：
--
作者：
Kohjima, M;Noda, Y;Sumimoto, H
从线虫到脊椎动物都保守的细胞极性蛋白PAR3与PAR6和非典型蛋白激酶C （aPKC）形成复合物，该蛋白复合物出现在哺乳动物上皮细胞的紧密连接处。在这里，我们克隆了一种新的PAR3同源物的人类cDNA，命名为PAR3 β，其信息存在于多种组织中，在成人和胎儿肾脏中表达最丰富。编码的蛋白含有1205个氨基酸，包含一个与PAR3alpha的apkc结合域同源的区域，这是另一个先前发现的人类同源物，以及三个PDZ结构域；PAR3alpha的第一个PDZ结构域被认为与PAR6相互作用。出乎意料的是，与在各种物种中发现的其他par3相比，par3 β不能与PAR6或aPKC的任何同型结合。然而，无论是内在表达还是外在表达，PAR3beta都定位于紧密连接，表明其定位不需要三元络合物的形成。(C) 2002 Elsevier Science （USA）。版权所有。
]The cell polarity protein PAR3, conserved from the nematode to the vertebrate, forms a complex with PAR6 and atypical protein kinase C (aPKC), and the protein complex occurs at the tight junctions in mammalian epithelial cells. Here we have cloned human cDNA for a novel PAR3 homologue, designated PAR3beta, whose messages are present in a variety of tissues and most abundantly expressed in the adult and fetal kidneys. The encoded protein of 1205 amino acids contains a region homologous to the aPKC-binding domain of PAR3alpha, another human homologue previously identified, and three PDZ domains; the first PDZ domain of PAR3alpha is considered to interact with PAR6. Unexpectedly, in contrast to other PAR3s found in various species, PAR3beta,is incapable of binding to any isotypes of PAR6 or aPKC. Nevertheless PAR3beta, expressed intrinsically or extrinsically, localizes to the tight junctions, indicating that the localization does not require the ternary complex formation. (C) 2002 Elsevier Science (USA). All rights reserved.