Proteome profiling of embryo chick retina
Proteome profiling of embryo chick retina
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DOI：
10.1186/1477-5956-6-3
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发表时间：
2008-01-22
期刊：
影响因子：
2
通讯作者：
Kiuchi, Yoshiaki
中科院分区：
文献类型：
--
作者：
Mizukami, Mina;Kanamoto, Takashi;Kiuchi, Yoshiaki
背景：目前对视网膜发育过程中的分子通路知之甚少。本研究的目的是鉴定可能参与视网膜发育的蛋白质。我们使用了蛋白质组学为基础的方法来确定蛋白质的上调或下调过程中的胚胎鸡retina.Results的发展：二维凝胶电泳进行与胚胎鸡，这是从胚胎的第7天（ED 7）和第11天（ED 11）的视网膜。选择显示显著差异的蛋白质点用于通过MALDI质谱法鉴定。在鸡胚视网膜中，有13个蛋白质在艾德11天时表达差异，其中7个蛋白质表达上调，6个蛋白质表达下调。还在胚胎第15天（ED 15）评价了显著的蛋白质。一些蛋白质被认为是调节细胞增殖，细胞死亡，运输，代谢，组织和细胞外基质，和其他人也包括新的proteins.Conclusion：我们确定了13个蛋白质差异表达的鸡胚胎视网膜在第7天，相比，视网膜的胚胎11天。它们是细胞信号传导的各种调节蛋白。
Background: Little is known regarding the molecular pathways that underlie the process of retinal development. The purpose of this study was to identify proteins which may be involved in development of retina. We used a proteomics-based approach to identify proteins that are up- or down-regulated during the development of the embryo chick retina.Results: Two-dimensional gel electrophoresis was performed with the retina of embryo chicken, which was obtained from embryos of day 7 ( ED7) and of day 11 ( ED11). The protein spots showing significant differences were selected for identification by MALDI mass spectrometry. Thirteen proteins were differentially expressed; seven proteins were up- regulated in embryo retina of chicken at ED 11 and six proteins were down-regulated. Significant proteins were also evaluated in embryo day 15 ( ED15). Some of identified proteins were known to regulate cell proliferation, cell death, transport, metabolism, organization and extracellular matrix, and others also included novel proteins.Conclusion: We identified thirteen proteins which differentially expressed in embryonal retina of chicken at day 7, as compared to the retina of embryo of day 11. They were various regulatory proteins for cellular signaling.