Separation and characterization of six (1 leads to 3)-beta-glucanases from Saccharomyces cerevisiae
Separation and characterization of six (1 leads to 3)-beta-glucanases from Saccharomyces cerevisiae
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酿酒酵母中六种（1 导致 3）-β-葡聚糖酶的分离和表征
DOI：
10.1128/jb.156.3.1204-1213.1983
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发表时间：
1983
影响因子：
3.2
通讯作者：
G. Fleet
中科院分区：
文献类型：
--
作者：
Nguyen H. HIENt;G. Fleet
利用DEAE-Bio-Gel、HTP-Bio-Gel和CM-Bio-Gel柱层析系统，从酿酒酵母单倍体菌株2180B的细胞壁自溶产物和细胞抽提液中分离和鉴定了6种不同的(1-到3)-β-葡聚糖酶。单倍体交配型酿酒酵母2180A和二倍体酿酒酵母2180D和595含有相同的葡聚糖酶复合体。葡聚糖酶II和IIIA为外切酶，葡聚糖酶I、IIIB、IV和V为内切酶。这些酶在分离的酵母细胞壁上表现出不同的相对分子质量、动力学性质和活性。用高压液相色谱对底物(海带多糖)的水解产物进行了定量，四种内切葡聚糖酶的产物有显著差异。
Using a system of chromatography through columns of DEAE-Bio-Gel, HTP-Bio-Gel, and CM-Bio-Gel, we isolated and characterized six different (1 leads to 3)-beta-glucanases from cell wall autolysates and cell extracts of Saccharomyces cerevisiae haploid strain 2180B. These enzymes were designated glucanases I, II, IIIA, IIIB, IV, and V. The haploid mating type S. cerevisiae strain 2180A and the diploid strains S. cerevisiae 2180D and S. cerevisiae 595 contained the same complex of glucanases. Glucanases II and IIIA were exoenzymes, and glucanases I, IIIB, IV, and V were endoenzymes. The enzymes exhibited different molecular weights, kinetic properties, and activities on isolated yeast cell walls. The products of substrate (laminarin) hydrolysis were quantified by using high-pressure liquid chromatography and were significantly different for the four endoglucanases.