Preservation of Bone Structure and Function by Lithothamnion sp. Derived Minerals
Preservation of Bone Structure and Function by Lithothamnion sp. Derived Minerals
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DOI：
10.1007/s12011-013-9820-7
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发表时间：
2013-12-01
影响因子：
3.9
通讯作者：
Varani, James
中科院分区：
文献类型：
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作者：
Aslam, Muhammad Nadeem;Bergin, Ingrid;Varani, James
进行性骨矿物质丢失和骨脆性增加是骨质疏松症的标志。研究了从红色海洋藻类石藻属中分离的矿物质组合抑制雌性小鼠骨矿物质损失的能力，所述雌性小鼠维持在标准啮齿动物食物（对照）饮食或高脂肪西方饮食（HFWD）5、12和18个月。在每个时间点，对股骨进行mu-CT分析和生物力学测试。还分析了尾椎的一个子集。随后，对骨骼中的各个元素进行了评估。抗酒石酸酸性磷酸酶（TRAP）和I型前胶原前肽（P1 NP）的5 b亚型的血清水平也进行了测量。两种饮食均发生骨小梁丢失（早在5个月时就明显）。皮质骨增加到第5个月，然后下降。皮质骨丢失主要发生在HFWD的小鼠中。在两种饮食中加入矿物质减少了骨矿物质的损失，并改善了骨强度。这些矿物质也增强了骨密度。在已知对骨骼健康重要的几种阳离子矿物质中，只有锶在喂食矿物质饮食的动物的骨组织中显著增加，但增加幅度很大（5-10倍）。在接受矿物质的小鼠中，TRAP的血清水平始终较高，但P1 NP的水平则不然。这些数据表明，从海洋红藻中提取的微量矿物质可与钙一起用于预防进行性骨矿物质流失。矿物质补充剂可以作为糖尿病预防策略的一部分。
Progressive bone mineral loss and increasing bone fragility are hallmarks of osteoporosis. A combination of minerals isolated from the red marine algae, Lithothamnion sp. was examined for ability to inhibit bone mineral loss in female mice maintained on either a standard rodent chow (control) diet or a high-fat western diet (HFWD) for 5, 12, and 18 months. At each time point, femora were subjected to mu-CT analysis and biomechanical testing. A subset of caudal vertebrae was also analyzed. Following this, individual elements were assessed in bones. Serum levels of the 5b isoform of tartrate-resistant acid phosphatase (TRAP) and procollagen type I propeptide (P1NP) were also measured. Trabecular bone loss occurred in both diets (evident as early as 5 months). Cortical bone increased through month 5 and then declined. Cortical bone loss was primarily in mice on the HFWD. Inclusion of the minerals in the diet reduced bone mineral loss in both diets and improved bone strength. Bone mineral density was also enhanced by these minerals. Of several cationic minerals known to be important to bone health, only strontium was significantly increased in bone tissue from animals fed the mineral diets, but the increase was large (5-10 fold). Serum levels of TRAP were consistently higher in mice receiving the minerals, but levels of P1NP were not. These data suggest that trace minerals derived from marine red algae may be used to prevent progressive bone mineral loss in conjunction with calcium. Mineral supplementation could find use as part of an osteoporosis-prevention strategy.