Neural stem-like cell line derived from a nonhematopoietic population of human umbilical cord blood
Neural stem-like cell line derived from a nonhematopoietic population of human umbilical cord blood
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DOI：
10.1089/scd.2006.15.391
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发表时间：
2006-06-01
影响因子：
4
通讯作者：
Domanska-Janik, Krystyna
中科院分区：
文献类型：
--
作者：
Buzanska, Leonora;Jurga, Marcin;Domanska-Janik, Krystyna
干细胞和祖细胞增殖和分化成其他谱系的能力被广泛认为是干细胞的特征。在此之前，我们已经报道了来自CD 34（-）（非造血）贴壁人脐带血亚群的细胞可以在体外获得多能神经祖细胞的特征。在本研究中，使用这些脐带血来源的干细胞，我们已经建立了一个克隆细胞系称为HUCB-NSCs（人脐带血神经干细胞），表达几种神经抗原，并已在培养中生长超过60代。在此期间，HUCB-NSC保持其生长速率，分化为神经元，星形胶质细胞和少突胶质细胞样细胞的能力，并显示稳定的核型。HUCB-NSC的DNA微阵列分析显示，与其他单核细胞相比，编码推定的干细胞和祖细胞标志物的选定基因的表达增强。dBcAMP诱导的HUCB-NSC进一步分化为更高级的神经元细胞。这是第一次报告的非转化HUCB-NSC线，可以在单层培养中连续生长，并诱导终末分化的建立和表征。这些细胞应该进一步了解参与神经干细胞自我更新和分化的调节机制。
The ability of stem and progenitor cells to proliferate and differentiate into other lineages is widely viewed as a characteristic of stem cells. Previously, we have reported that cells from a CD34(-) (non-hematopoietic) adherent subpopulation of human cord blood can acquire a feature of multipotential neural progenitors in vitro. In the present study, using these cord blood-derived stem cells, we have established a clonal cell line termed HUCB-NSCs ( human umbilical cord blood-neural stem cells) that expresses several neural antigens and has been grown in culture for more than 60 passages. During this time, HUCB-NSCs retained their growth rate, the ability to differentiate into neuronal, astrocyte-, and oligodendrocyte-like cells and displayed a stable karyotype. DNA microarray analysis of HUCB-NSCs revealed enhanced expression of selected genes encoding putative stem and progenitor cell markers when compared to other mononuclear cells. dBcAMP-induced HUCB-NSCs were further differentiated into more advanced neuronal cells. This is the first report of the establishment and characterization of a nontransformed HUCB-NSC line that can be grown continuously in a monolayer culture and induced to terminal differentiation. These cells should further our understanding of the regulatory mechanisms involved in NSC self-renewal and differentiation.