Correlative super-resolution fluorescence and electron microscopy of the nuclear pore complex with molecular resolution
Correlative super-resolution fluorescence and electron microscopy of the nuclear pore complex with molecular resolution
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DOI：
10.1242/jcs.156620
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发表时间：
2014-10-15
影响因子：
4
通讯作者：
Sauer, Markus
中科院分区：
文献类型：
--
作者：
Loeschberger, Anna;Franke, Christian;Sauer, Markus
Here, we combine super-resolution fluorescence localization microscopy with scanning electron microscopy to map the position of proteins of nuclear pore complexes in isolated Xenopus laevis oocyte nuclear envelopes with molecular resolution in both imaging modes. We use the periodic molecular structure of the nuclear pore complex to superimpose direct stochastic optical reconstruction microscopy images with a precision of
