A human peripheral blood monocyte-derived subset acts as pluripotent stem cells
A human peripheral blood monocyte-derived subset acts as pluripotent stem cells
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DOI：
10.1073/pnas.0536882100
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发表时间：
2003-03-04
影响因子：
11.1
通讯作者：
Huberman, E
中科院分区：
文献类型：
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作者：
Zhao, Y;Glesne, D;Huberman, E
我们从人类外周血单核细胞亚群中鉴定、培养、表征和繁殖成体多能干细胞 (PSC)。这些细胞在外观上类似于成纤维细胞，在巨噬细胞集落刺激因子存在下扩增，并显示单核细胞和造血干细胞标记物，包括 CD14、CD34 和 CD45。我们用脂多糖诱导这些细胞分化为成熟的巨噬细胞，用IL-2诱导T淋巴细胞，用表皮生长因子诱导上皮细胞，用血管内皮细胞生长因子诱导内皮细胞，用神经生长因子诱导神经元细胞，用肝细胞生长因子诱导肝细胞。克隆分析进一步证实了个体 PSC 的多能性。储存、扩增和区分来自自体外周血的 PSC 的能力应该使它们成为移植治疗的有价值的候选者。
We have identified, cultured, characterized, and propagated adult pluripotent stem cells (PSC) from a subset of human peripheral blood monocytes. These cells, which in appearance resemble fibroblasts, expand in the presence of macrophage colony-stimulating factor and display monocytic and hematopoietic stem cell markers including CD14, CD34, and CD45. We have induced these cells to differentiate into mature macrophages by lipopolysaccharide, T lymphocytes by IL-2, epithelial cells by epidermal growth factor, endothelial cells by vascular endothelial cell growth factor, neuronal cells by nerve growth factor, and liver cells by hepatocyte growth factor. The pluripotent nature of individual PSC was further confirmed by a clonal analysis. The ability to store, expand, and differentiate these PSC from autologous peripheral blood should make them valuable candidates for transplantation therapy.