In vitro Fertilization of Mouse Eggs in the Presence of Bovine Follicular Fluid
In vitro Fertilization of Mouse Eggs in the Presence of Bovine Follicular Fluid
复制标题
牛卵泡液存在下小鼠卵子的体外受精
作者：
T. Iwamatsu;M. Chang
自从在雌性生殖道中认识到“精子获能”以来，利用从子宫中提取的精子对兔子3,4、仓鼠5和小鼠进行了体外受精，并取得了成功。用附睾（无能力）仓鼠精子5，在有仓鼠7或母牛8的卵泡液存在的情况下，或在有兔输卵管囊肿液存在的情况下（我们未发表的工作），成功地在体外使金仓鼠卵受精，并在有金仓鼠卵泡液存在的情况下使中国仓鼠卵受精。鼓励我们进行以下实验，证明小鼠精子可以在体外使小鼠卵子在牛卵泡液的存在下受精。
SINCE the recognition of “capacitation of sperm” in the female tract1,2, fertilization in vitro of eggs has been successfully conducted by insemination of sperm recovered from the uterus for the rabbit3,4, hamster5 and mouse6. Successful fertilization of golden hamster eggs in vitro by epididymal (uncapacitated) hamster sperm5, in the presence of follicular fluid of hamster7 or cow8, or in the presence of rabbit tubal cyst fluid (our unpublished work), and that of Chinese hamster eggs in the presence of follicular fluid of golden hamster9, encouraged us to carry out the following experiment which demonstrated that mouse sperm can be capacitated in vitro to fertilize mouse eggs in the presence of bovine follicular fluid.