LeX is expressed by principle progenitor cells in the embryonic nervous system, is secreted into their environment and binds Wnt-1
LeX is expressed by principle progenitor cells in the embryonic nervous system, is secreted into their environment and binds Wnt-1
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DOI：
10.1016/j.ydbio.2005.12.030
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发表时间：
2006-03-15
影响因子：
2.7
通讯作者：
Temple, S
中科院分区：
文献类型：
--
作者：
Capela, A;Temple, S
LeX/SSEA1/CD15是一种细胞外基质相关碳水化合物，由胚胎干细胞、成人神经干细胞和骨髓干细胞表达。它对早期胚胎干细胞的细胞粘附、压实和FGF2应答具有重要意义；然而，其在后期的作用尚不清楚。我们现在发现LeX在小鼠原代神经祖细胞（包括神经干细胞、神经母细胞和胶质母细胞）中表达，而不是在它们的分化产物中表达。LeX区分了原始神经上皮中高度增殖的细胞，表明在放射状胶质细胞出现之前，细胞电位存在异质性。在后期，表达LeX的祖细胞在形态上通常呈放射状。表面LeX的表达可用于在FACS发育过程中富集来自不同中枢神经系统区域的神经干和祖细胞。我们发现LeX的表达在神经发生时间较长的神经区域，如嗅觉上皮、海马、基底前脑和小脑中尤为强烈。这些区域也表达高水平的生长因子FGF8和/或Wnt-1。我们在这里展示了发育中的神经系统中含有lex的分子与Wnt-1结合。我们的研究结果表明，存在于主要神经祖细胞表面并分泌到其细胞外生态位的LeX可能结合并呈递对其增殖和自我更新重要的生长因子。(C) 2005爱思唯尔公司版权所有。
LeX/SSEA1/CD15 is an extracellular matrix-associated carbohydrate expressed by ES cells and by adult neural and bone marrow stem cells. It is important for cell adhesion, compaction and FGF2 responses of early embryonic stem cells; however, its function at later stages is not clear. We now show that LeX is expressed by primary mouse neural progenitor cells, including neural stem cells, neuroblasts and glioblasts, but not by their more differentiated products. LeX distinguishes highly proliferative cells even in the primitive neuroepithelium, demonstrating heterogeneity in cell potential before radial glia arise. At later stages, LeX expressing progenitors are frequently radial in morphology. Surface LeX expression can be used to enrich neural stem and progenitor cells from different CNS regions throughout development by FACS. We found that LeX expression is particularly strong in neural regions with prolonged neurogenesis, e.g., the olfactory epithelium, hippocampus, basal forebrain and cerebellum. These regions also express high levels of the growth factors FGF8 and/or Wnt-1. We show here that LeX-containing molecules in the developing nervous system bind Wnt-1. Our findings suggest that LeX, which is present on the surface of principle neural progenitors and secreted into their extracellular niche, may bind and present growth factors important for their proliferation and self-renewal. (C) 2005 Elsevier Inc. All rights reserved.