Novel smooth muscle cell lines from transgenic mice harboring temperature-sensitive SV40 large T-antigen gene. Temperature-dependent expression of smooth muscle myosin heavy chain-1 and calponin genes.
Novel smooth muscle cell lines from transgenic mice harboring temperature-sensitive SV40 large T-antigen gene. Temperature-dependent expression of smooth muscle myosin heavy chain-1 and calponin genes.
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来自转基因小鼠的新型平滑肌细胞系，带有温度敏感的 SV40 大 T 抗原基因。
DOI：
10.1006/jmcc.1997.0453
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发表时间：
1997
影响因子：
5
通讯作者：
Y. Matsuda
中科院分区：
文献类型：
--
作者：
K. Hasegawa;E. Arakawa;S. Oda;N. Yanai;M. Obinata;Y. Matsuda
我们已经建立了新的血管平滑肌细胞系（SVS30和SVS24细胞），它们保留了平滑肌细胞的特异性标记，如α -肌动蛋白、平滑肌球蛋白重链-1和钙钙蛋白的表达，这些标记来自于含有温度敏感的SV40大t抗原基因的转基因小鼠。SVS细胞系表现出温度依赖性生长和SV40大t抗原的表达。有趣的是，在非允许温度（39℃）下培养的平滑肌肌球蛋白重链-1和钙钙蛋白的蛋白质和mRNA水平高于允许温度（33℃）。这些结果表明SV40大t抗原影响SVS细胞株平滑肌特异性标志物的表达，并且SVS细胞株的部分性状可以通过培养温度控制。SVS细胞系是研究平滑肌细胞表型调节调控、鉴定涉及平滑肌肌球蛋白重链-1和钙钙蛋白基因表达的平滑肌特异性转录因子的重要工具。
We have established novel vascular smooth muscle cell lines (SVS30 and SVS24 cells) which retain the expression of specific markers for smooth muscle cells, such as alpha-actin, smooth muscle myosin heavy chain-1, and calponin, from transgenic mice harboring the temperature-sensitive SV40 large T-antigen gene. SVS cell lines showed temperature-dependent growth and the expression of SV40 large T-antigen. Interestingly, protein and mRNA levels of smooth muscle myosin heavy chain-1 and calponin seen in culture at the non-permissive temperature (39 degrees C) were higher than those at the permissive temperature (33 degrees C). These results suggest that SV40 large T-antigen affects the expression of smooth muscle-specific markers in SVS cell lines, and that some of the characters in SVS cell lines can be controlled by culture temperature. SVS cell lines should be quite valuable tools with which to study the regulation of phenotypic modulation of smooth muscle cells, and to identify smooth muscle specific transcription factors which involve the expression of smooth muscle myosin heavy chain-1 and calponin genes.