Simultaneous Determination of Neoeriocitrin and Naringin in Rat Plasma After Oral Administration of a Chinese Compound Formulation by UPLC-MS-MS
Simultaneous Determination of Neoeriocitrin and Naringin in Rat Plasma After Oral Administration of a Chinese Compound Formulation by UPLC-MS-MS
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DOI：
10.1093/chromsci/48.5.342
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发表时间：
2010-05-01
影响因子：
1.3
通讯作者：
Li, Famei
中科院分区：
文献类型：
--
作者：
Li, Chao;Yang, Chunjuan;Li, Famei
建立了同时测定大鼠血浆中新圣草次苷和柚皮苷的液相色谱-串联质谱法。以橙皮苷为内标，采用甲醇沉淀蛋白质的方法制备血浆样品。在ACQUITY UPLC BEH C18色谱柱上进行分析，使用乙腈-水（20：80，v/v）作为移动的流动相。通过电喷雾电离质谱法在负离子模式下进行检测，并进行多反应监测。在15.0-960 ng/mL和12.0-1200 ng/mL的浓度范围内，新圣草次苷和柚皮苷的线性关系良好。日内和日间精密度分别为9.7%和7.6%的新圣草苷和7.8%和12.9%的柚皮苷。新圣草苷的准确度为-4.3%~0.43%，柚皮苷的准确度为-3.8%~3.0%。该方法已成功应用于中药复方骨疏丹口服后新圣草苷和柚皮苷在大鼠体内的药代动力学研究。
A sensitive, specific method has been developed for simultaneous determination of neoeriocitrin and naringin in rat plasma using liquid chromatography-tandem mass spectrometry. With hesperidin as the internal standard, plasma samples were prepared by protein precipitation with methanol. Analysis was carried out on an ACQUITY UPLC BEH C18 column using acetonitrile-water (20:80, v/v) as the mobile phase. Detection was performed by means of electrospray ionization mass spectrometry in negative ion mode with multiple reaction monitoring. Linear calibration curves of neoeriocitrin and naringin were obtained over the concentration ranges of 15.0-960 ng/mL and 12.0-1200 ng/mL, respectively. The intra- and inter-day precisions were within 9.7% and 7.6% for neoeriocitrin and 7.8% and 12.9% for naringin. The accuracy was from -4.3% to 0.43% for neoeriocitrin and from -3.8% to 3.0% for naringin. The validated method was successfully applied to the pharmacokinetic study of neoeriocitrin and naringin in rats after oral administration of a Chinese compound formulation, gushudan.