Prevalence of tick-borne encephalitis virus (TBEV) in samples of raw milk taken randomly from cows, goats and sheep in eastern Poland.
Prevalence of tick-borne encephalitis virus (TBEV) in samples of raw milk taken randomly from cows, goats and sheep in eastern Poland.
复制标题
波兰东部地区奶牛、山羊和绵羊随机抽取的生奶样本中蜱传脑炎病毒（TBEV）的患病率。
DOI：
--
复制
发表时间：
2010
影响因子：
1.7
通讯作者：
J. Dutkiewicz
中科院分区：
文献类型：
--
作者：
E. Cisak;A. Wójcik;V. Zając;J. Sroka;A. Buczek;J. Dutkiewicz
采用套式RT-PCR方法，对波兰东部森林脑炎高发区卢布林省8个牧场饲养的63头母牛、29只山羊和27只绵羊的119份未经巴氏灭菌的牛奶样品进行了森林脑炎病毒(TBEV)RNA的检测。牛奶样本也通过酶联免疫吸附试验检测抗-TBEV抗体的存在。RT-PCR检测结果显示，绵羊奶中的TBE病毒感染率最高(22.2%)，其次是山羊奶(20.7%)和奶牛奶(11.1%)。抗-TBEV抗体阳性率以绵羊奶最高(14.8%)，其次为奶牛奶(3.2%)和山羊奶(0%)。结果表明，在TBE流行地区饮用原料奶有感染TBEV的潜在风险，并表明在食用牛奶之前需要进行巴氏杀菌。
A total of 119 unpasteurized milk samples taken from 63 cows, 29 goats and 27 sheep bred on 8 farms situated on the territory of the Lublin province (eastern Poland), an area of risk of tick-borne encephalitis (TBE), were examined for the presence of RNA of tick-borne encephalitis virus (TBEV) by the nested RT-PCR method. Milk samples were also tested for the presence of anti-TBEV antibodies by ELISA test. By RT-PCR, the greatest prevalence of TBE virus was found in the milk of sheep (22.2%), followed by milk of goats (20.7%) and cows (11.1%). By ELISA, the greatest prevalence of anti- TBEV antibodies was found also in the milk of sheep (14.8%), followed by milk of cows (3.2%) and goats (0%). The results suggest a potential risk of infection with TBEV by drinking raw milk on endemic areas of TBE, and indicate a need for milk pasteurization before consumption.