Anti-Inflammatory Effects of Cumin Essential Oil by Blocking JNK, ERK, and NF-κB Signaling Pathways in LPS-Stimulated RAW 264.7 Cells.
Anti-Inflammatory Effects of Cumin Essential Oil by Blocking JNK, ERK, and NF-κB Signaling Pathways in LPS-Stimulated RAW 264.7 Cells.
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小茴香精油通过阻断 LPS 刺激的 RAW 264.7 细胞中 JNK、ERK 和 NF-kappa B 信号通路的抗炎作用
DOI：
10.1155/2015/474509
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Su H
中科院分区：
文献类型：
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作者：
Wei J;Zhang X;Bi Y;Miao R;Zhang Z;Su H
孜然种子（Cuminum cyminum L.）通常用于食品调味和香料。本研究采用从种子中提取的孜然精油（CuEO）对脂多糖（LPS）刺激的RAW 264.7细胞进行抗炎作用研究，并探讨其机制。通过GC-MS共鉴定出26种挥发性成分，其中含量最高的是对异丙基苯醛（48.773%）。线粒体呼吸依赖性3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑（MTT）还原试验表明，CuEO在所用浓度（0.0005 - 0.01%）下未表现出任何细胞毒性效应。实时荧光定量PCR检测显示，CuEO显著抑制诱导型一氧化氮合酶（iNOS）、环氧合酶（考克斯-2）、白细胞介素（IL-1）和IL-6的mRNA表达。Western blotting结果显示，CuEO可阻断LPS诱导的核因子-κ B（NF-κ B）的转录激活，抑制细胞外信号调节激酶（ERK）和c-Jun N-末端激酶（JNK）的磷酸化。这些结果表明，CuEO通过抑制NF-κ B和丝裂原活化蛋白激酶ERK和JNK信号通路在LPS刺激的RAW 264. 7细胞中发挥抗炎作用;该化学品可用作抗炎剂的来源以及用于健康促进的膳食补充剂。
Cumin seeds (Cuminum cyminum L.) have been commonly used in food flavoring and perfumery. In this study, cumin essential oil (CuEO) extracted from seeds was employed to investigate the anti-inflammatory effects in lipopolysaccharide- (LPS-) stimulated RAW 264.7 cells and the underlying mechanisms. A total of 26 volatile constituents were identified in CuEO by GC-MS, and the most abundant constituent was cuminaldehyde (48.773%). Mitochondrial-respiration-dependent 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) reduction assay demonstrated that CuEO did not exhibit any cytotoxic effect at the employed concentrations (0.0005–0.01%). Real-time PCR tests showed that CuEO significantly inhibited the mRNA expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), interleukin- (IL-) 1, and IL-6. Moreover, western blotting analysis revealed that CuEO blocked LPS-induced transcriptional activation of nuclear factor-kappa B (NF-κB) and inhibited the phosphorylation of extracellular signal regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). These results suggested that CuEO exerted anti-inflammatory effects in LPS-stimulated RAW 264.7 cells via inhibition of NF-κB and mitogen-activated protein kinases ERK and JNK signaling; the chemical could be used as a source of anti-inflammatory agents as well as dietary complement for health promotion.