Dynamics of transitional endoplasmic reticulum sites in vertebrate cells
Dynamics of transitional endoplasmic reticulum sites in vertebrate cells
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DOI：
10.1091/mbc.11.9.3013
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发表时间：
2000-09-01
影响因子：
3.3
通讯作者：
Glick, BS
中科院分区：
文献类型：
--
作者：
Hammond, AT;Glick, BS
一个典型的脊椎动物细胞含有数百个过渡性内质网(TER)。推测，TER位点产生内质网高尔基体中间隔(ERGIC)的元件，ERGIC元件然后产生高尔基体池。因此，刻画影响TER分布的机制可能有助于揭示高尔基体的动态行为。我们以Sec13作为标记蛋白，对TER位点的性质进行了研究。荧光显微镜证实，TER位点是长寿命的ER亚域。TER位点在间期增殖，但在有丝分裂时丢失Sec13。与ERGIC元素不同，TER站点移动很少。然而，当微管被诺康唑解聚时，TER位点迅速重新分布，在高尔基结构旁边形成簇。因此，TER网站的不同寻常之处在于它是固定的，但又是动态的。这些发现可以用一个模型来解释，在这个模型中，来自高尔基体的逆行膜交通创造了新的TER位点。我们认为，特-高尔基系统是由这两个隔室之间的相互反馈组成的。
A typical vertebrate cell contains several hundred sites of transitional ER (tER). Presumably, tER sites generate elements of the ER-Golgi intermediate compartment (ERGIC), and ERGIC elements then generate Golgi cisternae. Therefore, characterizing the mechanisms that influence tER distribution may shed light on the dynamic behavior of the Golgi. We explored the properties of tER sites using Sec13 as a marker protein. Fluorescence microscopy confirmed that tER sites are long-lived ER subdomains. tER sites proliferate during interphase but lose Sec13 during mitosis. Unlike ERGIC elements, tER sites move very little. Nevertheless, when microtubules are depolymerized with nocodazole, tER sites redistribute rapidly to form clusters next to Golgi structures. Hence, tER sites have the unusual property of being immobile, yet dynamic. These findings can be explained by a model in which new tER sites are created by retrograde membrane traffic from the Golgi. We propose that the tER-Golgi system is organized by mutual feedback between these two compartments.