Effect of androgen and thyroid hormones on renin-1 messenger ribonucleic acid levels in mouse submandibular gland.
Effect of androgen and thyroid hormones on renin-1 messenger ribonucleic acid levels in mouse submandibular gland.
复制标题
雄激素和甲状腺激素对小鼠颌下腺肾素-1信使核糖核酸水平的影响。
DOI：
10.1016/0303-7207(88)90070-6
复制
发表时间：
1988
影响因子：
4.1
通讯作者：
Wilson,CM
中科院分区：
文献类型：
--
作者：
McGowan,RA;Gross,KW;Wilson,CM
小鼠下颌下腺(SMG)颗粒曲管细胞中肾素和其他生物活性多肽的合成受雄激素和甲状腺激素的调节。本研究以携带单个肾素结构基因(REN-1)的遗传性甲状腺功能低下(HYT/HYT)小鼠为研究对象，探讨激素在SMG中的作用机制。Northern分析表明，用5α-双氢睾酮(DHT)和/或甲状腺素(T4)处理雌性小鼠可增强肾素-1活性，并增加肾素-1mRNA的表达。与正常甲状腺(HYT/+)小鼠相比，HYT/hyt小鼠的肾素-1mRNA基础水平较低，且仅对任何一种激素的反应迟钝。DHT和T4协同作用，可增加hyt/hyt雌鼠SMG的肾素-1活性和肾素-1mRNA的表达。此外，肾素-1活性和肾素-1mRNA水平在这些动物的SMG中的变化是一致的。这些数据表明，雄激素和甲状腺激素主要通过调节可供翻译的肾素-1mRNA的量来影响小鼠SMG中肾素-1的水平。
The synthesis of renin and other biologically active polypeptides in the granular convoluted tubule cells of the mouse submandibular gland (SMG) is regulated by androgen and thyroid hormones. In this study genetically hypothyroid (hyt/hyt) mice carrying a single renin structural gene (Ren-1) were used to investigate the mechanism of hormonal action in mouse SMG. Treatment of female mice with 5α-dihydrotestosterone (DHT) and/or thyroxine (T4) enhanced renin-1 activity and increased renin-1 mRNA, determined by Northern analysis. Compared to euthyroid (hyt/ +) littermates,hyt/hytmice had lower basal levels of renin-1 mRNA and a blunted response to either hormone alone. DHT and T4acted synergistically to increase renin-1 activity and renin-1 mRNA in the SMG ofhyt/hytfemales. Furthermore, levels of renin-1 activity and renin-1 mRNA varied concordantly in the SMG of these animals. These data indicate that androgen and thyroid hormones influence levels of renin-1 in mouse SMG primarily by regulating the amount of renin-1 mRNA available for translation.