Characterization of retroviruses from patients with multiple sclerosis
Characterization of retroviruses from patients with multiple sclerosis
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DOI：
10.1111/j.1600-0404.1997.tb08150.x
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发表时间：
1997-01-01
影响因子：
3.5
通讯作者：
MollerLarsen, A
中科院分区：
文献类型：
--
作者：
Christensen, T;Jensen, AW;MollerLarsen, A
进行这些研究以表征在从6名多发性硬化症患者、1名进行性脊髓病患者和1名健康对照的外周血单核细胞（PBMNC）自发发育的细胞系中发现的逆转录病毒。这些细胞系是B淋巴母细胞样的，并产生EB病毒（EBV）颗粒或表达EBV蛋白。B-淋巴母细胞样细胞系的特征还在于产生低的、波动量的逆转录病毒。低生产率使纯化和表征复杂化，但产物增强型逆转录酶（PERT）测定的实施为监测逆转录病毒生产提供了非常有用的工具。通过电子显微镜，逆转录病毒颗粒看起来像C型。功能测定表明存在Pol、Gag和Env。间接ELISA证明了疾病活动性和对逆转录病毒肽的反应性之间的显着关系。分子表征主要基于RT-PCR、克隆、测序和北方或南方分析。分子表征仍在继续。
These studies were performed to characterize retroviruses found in cell lines spontaneously developed from peripheral blood mononuclear cells (PBMNC) from 6 multiple sclerosis patients, a patient with progressive myelopathy and a healthy control. The cell lines are B-lymphoblastoid and produce Epstein-Barr virus (EBV) particles or express EBV proteins. The B-lymphoblastoid cell lines are also characterized by production of low, fluctuating amounts of retrovirus. The low productivity complicates purification and characterization, but implementation of product-enhanced reverse transcriptase (PERT) assays has provided a highly useful tool for monitoring retrovirus production. By electron microscopy, the retroviral particles appear type-C-like. Functional assays indicate the presence of Pol, Gag and Env. Indirect ELISA demonstrates a significant relation between disease activity and reactivity towards retroviral peptides. Molecular characterization is primarily based on RT-PCR, cloning, sequencing and Northern- or Southern analyses. Molecular characterization is continuing.