Maternal alcohol exposure before and during pregnancy: effect on development of neurons and glial cells in culture.
Maternal alcohol exposure before and during pregnancy: effect on development of neurons and glial cells in culture.
复制标题
怀孕前和怀孕期间的母亲酒精暴露：对培养物中神经元和神经胶质细胞发育的影响。
作者：
M. Ledig;L. Megias;G. Tholey
在体外培养的胎鼠脑神经元和神经胶质细胞上，观察了孕期酒精暴露对神经细胞发育的影响。从两周大的皮质脑细胞培养神经元一周，从新生皮质脑细胞培养神经胶质细胞四周。进行了两种类型的母亲酒精治疗；要么是在怀孕前和怀孕期间，要么是在怀孕开始之前。在这两种情况下，我们发现通过显微镜观察和神经细胞发育的酶标记物(神经元细胞的非神经元烯醇化酶和神经元特异性烯醇化酶，神经胶质细胞的非神经元烯醇化酶和谷氨酰胺合成酶)的测定来评估神经细胞成熟延迟。这一结果证实了我们之前的体内实验，指出了母亲酒精暴露对后代的长期影响。
The effect of maternal alcohol exposure on nerve cell development was investigated in neurons and glial cells cultured from foetal rat brain. Neurons were grown for one week from two-week-old cortical brain cells and glial cells were cultured for four weeks from newborn cortical brain cells. Two types of maternal alcohol treatment were performed; either before and during pregnancy or only until the beginning of pregnancy. In both situations, we found a delayed nerve cell maturation assessed by microscopic observations and determination of enzymatic markers of nerve cell development (non-neuronal and neuron-specific enolase for the neuronal cells, non-neuronal enolase and glutamine synthetase for the glial cells). The results confirmed our previous in vivo experiments pointing out long-lasting effects of maternal alcohol exposure in the offspring.