THE HEMATOPOIETIC GROWTH FACTOR-KL IS ENCODED BY THE SI-LOCUS AND IS THE LIGAND OF THE C-KIT RECEPTOR, THE GENE-PRODUCT OF THE W-LOCUS
THE HEMATOPOIETIC GROWTH FACTOR-KL IS ENCODED BY THE SI-LOCUS AND IS THE LIGAND OF THE C-KIT RECEPTOR, THE GENE-PRODUCT OF THE W-LOCUS
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DOI：
10.1016/0092-8674(90)90303-v
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发表时间：
1990-10-05
期刊：
影响因子：
64.5
通讯作者：
BESMER, P
中科院分区：
文献类型：
--
作者：
HUANG, E;NOCKA, K;BESMER, P
小鼠钢基因座（SI）的突变与白色斑点基因座（W）的突变影响相同的细胞靶点，W与c-kit原癌基因是等位基因。我们表明，KL，从BALB/c 3 T3成纤维细胞，刺激肥大细胞和早期红系祖细胞的增殖的条件培养基中获得的造血生长因子，特异性结合到c-kit受体。预测的氨基酸序列的分离KL特异性cDNA克隆表明，KL是作为一个完整的跨膜蛋白合成。连锁分析将KL基因定位于小鼠10号染色体上的SI基因座，并且KL序列在SI小鼠的基因组中缺失。这些结果表明，SI基因座编码c-kit受体KL的配体。
Mutations at the steel locus (SI) of the mouse affect the same cellular targets as mutations at the white spotting locus (W), which is allelic with the c-kit proto-oncogene. We show that KL, a hematopoietic growth factor obtained from conditioned medium of BALB/c 3T3 fibroblasts that stimulates the proliferation of mast cells and early erythroid progenitors, specifically binds to the c-kit receptor. The predicted amino acid sequence of isolated KL-specific cDNA clones suggests that KL is synthesized as an integral transmembrane protein. Linkage analysis maps the KL gene to the SI locus on mouse chromosome 10, and KL sequences are deleted in the genome of the SI mouse. These results indicate that the SI locus encodes the ligand of the c-kit receptor, KL.