Nitric oxide acts as a retrograde messenger during long-term potentiation in cultured hippocampal neurons
Nitric oxide acts as a retrograde messenger during long-term potentiation in cultured hippocampal neurons
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DOI：
10.1016/s0928-4257(97)87907-7
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发表时间：
1996-01-01
影响因子：
--
通讯作者：
Hawkins, RD
中科院分区：
文献类型：
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作者：
Arancio, O;LevRam, V;Hawkins, RD
我们研究了长时程增强（LTP）在突触前，突触后，或细胞外应用一氧化氮（NO）清除剂，一氧化氮合酶的抑制剂，和膜不渗透的NO供体，释放NO后，只与UV光光解分离的细胞培养海马神经元之间的突触。我们的研究结果表明，NO产生于突触后神经元，通过细胞外空间，并直接作用于突触前神经元产生长时程增强，支持这一假设，即NO在LTP过程中充当逆行信使。
We examined long-term potentiation (LTP) at synapses between hippocampal neurons in dissociated cell culture following presynaptic, postsynaptic, or extracellular application of a nitric oxide (NO) scavenger, an inhibitor of NO synthase, and a membrane-impermeant NO donor that releases NO only upon photolysis with UV light. Our results indicate that NO is produced in the postsynaptic neuron, travels through the extracellular space, and acts directly in the presynaptic neuron to produce long-term potentiation, supporting the hypothesis that NO acts a retrograde messenger during LTP.