Quiescent and activated mouse granulocytes do not express granzyme A and B or perforin: similarities or differences with human polymorphonuclear leukocytes?
Quiescent and activated mouse granulocytes do not express granzyme A and B or perforin: similarities or differences with human polymorphonuclear leukocytes?
复制标题
静止和激活的小鼠粒细胞不表达颗粒酶 A 和 B 或穿孔素：与人多形核白细胞的相似性或差异？
作者：
P. Martin;R. Wallich;J. Pardo;A. Müllbacher;M. Munder;M. Modolell;M. Simon
多态性白细胞已被证明使用多种效应子功能来对抗病原体和肿瘤，包括酶、防御素和毒性产物如氧自由基和氮氧化物。最近的研究为粒酶（gzms）和穿孔素（perf）在人类中性粒细胞的细胞毒武器库中的表达提供了证据，其有效性受到随后2项研究的质疑。我们现在已经使用细胞学，细胞内流式细胞术，酶分析，免疫电镜，和定量逆转录酶-聚合酶链反应，以获得证据的存在gzms和/或perf在小鼠Gr-1+粒细胞群体。获得的数据清楚地表明，无论是在体外还是在体内衍生的小鼠粒细胞合成gzmA和gzmB或perf，即使在感染/免疫与病原体或病原体衍生的材料。对来自3名健康对照受试者和4名患有不同疾病的患者的人中性粒细胞中gzmB表达的平行可比分析未能检测到gzmB表达。数据表明，来自小鼠和人的多形核白细胞缺乏3种细胞毒性效应分子gzmA、gzmB和perf，通常与自然杀伤和细胞毒性T淋巴细胞相关。
Polymorphonuclear leukocytes have been shown to use a multitude of effector functions to combat pathogens and tumors, including enzymes, defensins, and toxic products such as oxygen radicals and nitrogen oxides. Recent studies provided evidence for the expression of granzymes (gzms) and perforin (perf) within the cytotoxic arsenal of human neutrophils, the validity of which was questioned by 2 subsequent studies. We have now used cytology, intracellular flow cytometry, enzymatic assays, immunoelectron microscopy, and quantitative reverse transcriptase-polymerase chain reaction to obtain evidence of the presence of gzms and/or perf in mouse Gr-1+ granulocyte populations. The data obtained clearly demonstrate that neither in vitro- nor in vivo-derived mouse granulocytes synthesize gzmA and gzmB or perf, even following infection/immunization with pathogens or pathogen-derived material. A parallel comparable analysis on the expression of gzmB in human neutrophils from 3 healthy control subjects and 4 patients with diverse diseases failed to detect gzmB expression. The data indicate that polymorphonuclear leukocytes from mice and humans lack the 3 cytotoxic effector molecules, gzmA, gzmB, and perf, generally associated with natural killer and cytotoxic T lymphocytes.
影响因子：
4.5
作者：
KAGAN, BL;GANZ, T;LEHRER, RI
通讯作者：
LEHRER, RI
影响因子：
2.2
作者：
Lagasse, E;Weissman, IL
通讯作者：
Weissman, IL