The aberrant expression and localization of prohibitin during apoptosis of human cholangiocarcinoma Mz‐ChA‐1 cells
The aberrant expression and localization of prohibitin during apoptosis of human cholangiocarcinoma Mz‐ChA‐1 cells
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DOI：
10.1016/j.febslet.2013.12.021
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发表时间：
2014-01
期刊：
影响因子：
3.5
通讯作者：
Wei Song;L. Tian;Shan-Shan Li-Shan;Dong-Yan Shen;Qing-Xi Chen
中科院分区：
文献类型：
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作者：
Wei Song;L. Tian;Shan-Shan Li-Shan;Dong-Yan Shen;Qing-Xi Chen
本研究旨在探讨在人胆管癌细胞凋亡过程中，抑制素(PHB)的异常表达和定位变化。我们的研究表明，PHB主要在细胞质中表达，在细胞核中只有少量表达。但在细胞凋亡过程中，PHB的表达显著降低，其定位由胞浆移至胞核。PHB与AIF、Rb、P53、c-Fos共定位，但治疗后共定位区域发生改变。同时，我们在Mz-CHA-1细胞中检测到PHB与P53和RB之间存在直接的相互作用。这些结果表明，PHB定位和表达的改变，以及它与相关癌基因和抑癌基因的共定位，可以影响Mz-CHA-1细胞的凋亡。
In this study, we aimed to investigate the aberrant expression and shift in localization of prohibitin (PHB) during apoptosis of human cholangiocarcinoma cells. Our study demonstrated that PHB was expressed primarily in the cytoplasm and only a little in the nucleus. However, PHB expression significantly decreased, and its localization shifted from the cytoplasm to the nucleus during apoptosis. PHB co-localized with AIF, Rb, p53, and c-Fos, but the region of co-localization was altered after treatment. Meanwhile, we detected a direct interaction between PHB and both p53 and Rb in Mz-ChA-1 cells. These results suggest that the altered localization and expression of PHB, as well as its co-localization with related oncogenes and tumor suppressor genes, can affect the apoptosis of Mz-ChA-1 cells.