NK phenotypic markers and IL2 response in NK cells from elderly people
NK phenotypic markers and IL2 response in NK cells from elderly people
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DOI：
10.1016/s0531-5565(98)00076-x
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发表时间：
1999-04-01
影响因子：
3.9
通讯作者：
Solana, R
中科院分区：
文献类型：
--
作者：
Borrego, F;Alonso, MC;Solana, R
免疫衰老是一个主要影响免疫系统T细胞的过程，尽管与年龄相关的免疫学改变也被证明存在于NK细胞的表型和功能中。随着年龄的增长，NK细胞的数量显著增加。所有外周血淋巴细胞的NK细胞毒能力也得到了很好的保存，不仅在健康的老年人，在百岁老人中也是如此。然而，当以每个细胞为单位考虑时，NK细胞对K562的杀伤作用是受损的，这种缺陷与激活后的信号转导缺陷有关，而不是与偶联形成或杀伤能力的降低有关。我们研究了符合老年人标准的老年供者的NK细胞表型。我们还研究了当考虑到不同的NR细胞功能时，这些细胞被IL2激活的能力，而不是细胞毒性。我们的结果证实了老年人NK细胞百分比的增加是由于CD56(DIM)亚群的扩大，这也显示了激活标记的变化模式，而CD56(BIT)亚群没有发现差异。当考虑细胞增殖、CD69表达和钙动员时，NK细胞对IL2的反应受到损害，而对TNF-α的产生没有显著影响。这些结果表明，尽管衰老对不同的NK细胞生物学功能有不同的影响，从严重的损害到可以忽略的损害，取决于所考虑的参数，但这些结果表明，人NK细胞并不能逃脱衰老过程。(C)1999 Elsevier Science Inc.保留所有权利。
Immunosenescence is a process that primarily affects the T cell compartment of the immune system, although age-associated immunological alterations have: also been demonstrated in the NK cell phenotype and function. A significant expansion in the number of NK cells is found in aging. The NK cytotoxic capacity of total peripheral blood lymphocytes is also well preserved, not only in healthy elderly people but also in centenarians. However, NK cell killing of K562 is impaired when considered in a per-cell basis, and this defect is associated with defective signal transduction after activation more than a diminished conjugate formation or killing capacity. We have studied the phenotype of NK cells in elderly donors fulfilling the Senieur criteria. We have also studied the capacity of these cells to be activated by IL2 when different NR cell functions, other than cytotoxicity, are considered. Our results confirm the increased percentage of NK cells in the elderly due to the expansion of the CD56(dim) subset that also show an altered pattern of activation markers, whereas no differences were found in the CD56(britght) subset. The response of NK cells to IL2 was found to be impaired when proliferation, expression of CD69, and Ca2+ mobilization were considered, whereas TNF-alpha production was not significantly affected, These results suggest that human NK cells do not escape the aging process, although senescence have a differential effect on distinct NK cell biological functions, ranging from severe to negligible impairment, depending on the parameters considered. (C) 1999 Elsevier Science Inc. All rights reserved.