Chromosomal and plasmid locations for phosphoribulokinase genes in Alcaligenes eutrophus
Chromosomal and plasmid locations for phosphoribulokinase genes in Alcaligenes eutrophus
复制标题
真养产碱菌中磷酸核糖激酶基因的染色体和质粒位置
作者：
R. Klintworth;M. Husemann;J. Salnikow;B. Bowien
编码磷酸核糖激酶（PRK）的基因是卡尔文循环的关键酶，位于化能自养生物真养产碱菌的基因组中。确定了 PRK 亚基的 NH2 末端序列。利用与该序列的一部分互补的合成寡脱氧核苷酸探针，杂交分析显示 PRK 基因位于真养放线菌 H16 的染色体和巨质粒 pHG1 上。
Genes coding for phosphoribulokinase (PRK), a key enzyme of the Calvin cycle, were localized in the genome of the chemoautotroph Alcaligenes eutrophus. The NH2-terminal sequence of the PRK subunit was determined. With a synthetic oligodeoxynucleotide probe complementary to a portion of this sequence, hybridization analysis revealed PRK genes to be located on both the chromosome and the megaplasmid pHG1 of A. eutrophus H16.