ADP RIBOSYL CYCLASE ACTIVITY OF A NOVEL BONE-MARROW STROMAL CELL-SURFACE MOLECULE, BST-1
ADP RIBOSYL CYCLASE ACTIVITY OF A NOVEL BONE-MARROW STROMAL CELL-SURFACE MOLECULE, BST-1
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DOI：
10.1016/0014-5793(94)01279-2
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发表时间：
1994-12-19
期刊：
影响因子：
3.5
通讯作者：
HIRANO, T
中科院分区：
文献类型：
--
作者：
HIRATA, Y;KIMURA, N;HIRANO, T
人BST-1是一种骨髓基质细胞表面分子，是一种促进前B细胞生长的GPI锚定蛋白。推导的人和小鼠BST-1的氨基酸序列与CD 38和Astrasia ADP核糖基环化酶的氨基酸序列有30%左右的同源性。因此，BST-1可能与CD 38一样具有ADP核糖基环化酶活性。在这里，我们报告建立一个稳定的CHO细胞系，它分泌截短的人可溶性BST-1，并显示纯化的可溶性BST-1显示ADP核糖环化酶和cADPR水解酶活性。
Human BST-1, a bone marrow stromal cell surface molecule, is a GPI-anchored protein that facilitates the growth of pre-B cells. The deduced amino acid sequences of human and mouse BST-1 show around 30% homology with those of CD38 and Aplysia ADP ribosyl cyclase. Therefore, like CD38, BST-1 might possess ADP ribosyl cyclase activity. Here, we report the establishment of a stable transformant CHO cell line, which secretes truncated human soluble BST-1, and show that purified soluble BST-1 displays both ADP ribosyl cyclase and cADPR hydrolase activities.