Ultrasonic-Assisted Preparation, Characterization and Antibacterial Activity of β-Chitosan from Squid Pens
Ultrasonic-Assisted Preparation, Characterization and Antibacterial Activity of β-Chitosan from Squid Pens
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鱿鱼圈β-壳聚糖的超声辅助制备、表征及抗菌活性
DOI：
10.4028/www.scientific.net/amr.236-238.282
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Qianfei Shao
中科院分区：
文献类型：
--
作者：
Jun Huang;Jian;Sheng Hu;Dongke Zhao;L. Mei;Shi Wang Liu;Y. Wu;Sheng Fang;Qianfei Shao
以乌贼笼为原料，采用超声波辅助NaOH溶液制备β-壳聚糖。采用单因素实验和L9（34）正交实验研究了反应时间、NaOH浓度和反应温度对β-甲壳素脱乙酰化反应的影响。甲壳素脱乙酰的最佳工艺条件为：反应温度80°C，反应时间2 h，NaOH浓度50%。在此条件下，β-甲壳素的脱乙酰度可达71.32%~ 92.91%。通过傅里叶变换红外光谱法证实了鱿鱼圈中含有β-壳聚糖。制备的β-壳聚糖对金黄色葡萄球菌（S.金黄色葡萄球菌（aureus）和大肠杆菌（E.coli）的最小抑菌浓度（MIC）进行比较。结果表明，不同脱乙酰度的β-壳聚糖具有不同的抗菌活性。S.制备的β-壳聚糖对金黄色葡萄球菌的抑菌作用强于大肠杆菌。
β-chitosan preparation from squid pens was carried out using aqueous NaOH with the ultrasonic assistance. Single factor experiments and L9 (34) orthogonal experiments were used to investigate the effect of three parameters (reaction time, concentration of NaOH and reaction temperature) on deacetylation of β-chitin. The optimal conditions for deacetylation of chitin were reaction temperature 80°C, reaction time 2 h and concentration of NaOH 50%. The optimal conditions allowed deacetylation degree of β-chitin from 71.32% to 92.91%. The β-chitosan from squid pens was confirmed by Fourier transform infrared spectroscopy. The antibacterial activities of the prepared β-chitosans again staphylococcus aureus (S. aureus) and Escherchia coli (E.coli) were then determined and compared by the MIC (minimum inhibitory concentration). Results indicate that β-chitosans with different degrees of deacetylation (DD) possess different antibacterial activity. The growth of S. aureus can be easily inhibited by prepared β-chitosan than E.coli.