Engineering of protein crystals for development of bionanomaterials
Engineering of protein crystals for development of bionanomaterials
复制标题
DOI：
10.7567/1347-4065/ab1399
复制
发表时间：
2019-06
影响因子：
1.5
通讯作者：
Tien Khanh Nguyen;Thuc Toan Pham;T. Ueno
中科院分区：
文献类型：
--
作者：
Tien Khanh Nguyen;Thuc Toan Pham;T. Ueno
蛋白质晶体是通过蛋白质单体的高度有序堆积形成的，它可以与超分子网络形成组装。组装结构固态中氨基酸残基的典型排列使具有特定晶格的不同环境得以形成。工程晶体蛋白质组装体已被证明是开发具有离散性质、结构和功能特征的材料的一种很有前途的方法。通过特定位点突变、金属共轭和二硫键的产生进行修饰的蛋白质晶体有助于构建各种复合材料。本文综述了在体外和体内设计蛋白质晶体的研究进展，目的是开发生物材料在生物科学和生物技术中的应用。
Protein crystals are formed with highly ordered packing of protein monomers, which can form assemblies with supramolecular networks. The typical alignment of amino acid residues in the solid state of the assembly structures enables the formation of different environments with specific lattices. Engineering crystalline protein assemblies has been demonstrated as a promising method for developing materials with discrete properties, structures and functional features. Protein crystals engineered with modification provided by specific site mutations, metal conjugation and generation of disulfide bonds have contributed to the construction of various composites. This article provides a review of progress in efforts to engineer in vitro and in vivo protein crystals with the objective of developing bionanomaterials for applications in bioscience and biotechnology.