Flow cytometry-based analysis by Sysmex-UF1000i® is an alternative method in the assessment of periodontal inflammation
Flow cytometry-based analysis by Sysmex-UF1000i® is an alternative method in the assessment of periodontal inflammation
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DOI：
10.1016/j.cca.2014.05.021
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发表时间：
2014-09-25
影响因子：
5
通讯作者：
Delanghe, Joris R.
中科院分区：
文献类型：
--
作者：
Coopman, Renaat;Speeckaert, Marijn M.;Delanghe, Joris R.
背景：牙周炎是一种常见的炎症状态。方法：对249名健康成人(年龄18~81岁；2.5~97.5百分位数：19~66岁)进行龋病、菌斑和牙周炎的调查。在Sysmex UF-1000i(R)上用流式细胞仪分析唾液。结果：Sysmex UF-1000i(R)能够重现唾液细胞成分的可靠测量。与健康成人相比，牙周炎患者的唾液细菌数量显著减少(p<；0.0001)。不同牙龈指数评分的患者唾液白细胞计数有显著差异(p<；0.0005)。牙周炎评分与唾液白细胞数、患者年龄、龋齿和牙菌斑程度密切相关(r(2)=0.6，p<；0.001)。以10(3)个白细胞/亩L唾液为界值，对牙周炎评分为3分的患者，其灵敏度为76%，特异度为78%，曲线下面积为0.82。结论：流式细胞术是评价口腔局部炎症过程的一种替代方法，灵敏度为76%，特异度为78%。(C)2014爱思唯尔B.V.保留所有权利。
Background: Gingivitis is a common inflammatory condition. We explored the value of flow cytometry of saliva in patients with periodontal inflammation.Methods: A cohort of 249 healthy adults (age range: 18-81 y; 2.5th to 97.5th percentile: 19-66 y) was investigated for caries, dental plaque and gingivitis. Saliva was analyzed using flow cytometry on a Sysmex UF-1000i (R).Results: Sysmex UF-1000i (R) is capable to reproduce reliable measurements of cellular components in saliva. A statistically significant lower number of salivary bacteria was found in patients with gingivitis in comparison with healthy adults (p < 0.0001). A significant difference in salivary leukocyte count was found between patients with different gingival index scores (p < 0.0005). The gingivitis score was strongly dependent on the number of salivary leukocytes, the age of the patient and the degree of caries and dental plaque (r(2) = 0.60, p < 0.001). At a cut-off level of 10(3) leukocytes/mu l saliva, an area under the curve of 0.82 was obtained with a sensitivity of 76% and a specificity of 78% in patients (>35 y) with a gingivitis score of 3.Conclusion: Flow cytometry is an alternative method to evaluate local inflammatory processes in the mouth with a sensitivity of 76% and a specificity of 78%. (C) 2014 Elsevier B.V. All rights reserved.