Silver stains demonstrating neuroendocrine cells
Silver stains demonstrating neuroendocrine cells
复制标题
DOI：
10.1080/10520290410001715264
复制
发表时间：
2004-02-01
影响因子：
1.6
通讯作者：
Grimelius, L
中科院分区：
文献类型：
--
作者：
Grimelius, L
在前免疫组织化学时代，银染剂是染色工具的重要组成部分，用于识别组织切片中的某些组织结构和细胞类型。其中一些可用于展示内分泌细胞，尤其是胃肠道中的内分泌细胞。直到 20 世纪 50 年代末，银染色，特别是那些识别内分泌细胞的银染色，都伴随着由于染色因素不受控制而导致的许多技术困难。 20 世纪 60 年代，针对内分泌细胞类型开发了新的银染色剂，这些染色剂给出了可重复的结果。本演示强调了一种“较旧”的银染技术和两种“较新”的银染技术，即 Masson、Grimelius 和 Sevier-Munger 技术。 Masson 染色显示肠嗜铬细胞（EC，血清素），Grimelius 染色是广泛的内分泌细胞标记物，Sevier-Munger 技术显示 EC 和 EC 样细胞以及甲状腺 C 细胞。特别是在前免疫组织化学时代，这些染色方法经常用于组织病理学诊断，特别是Grimelius技术。银染剂是根据经验开发的，除了少数例外，其化学背景尚不清楚。包括染色方案。
During the preimmunohistochemical era, silver stains were an important part of the staining arsenal for identifying certain tissue structures and cell types in tissue sections. Some of them were useful for demonstrating endocrine cells, especially in the gastrointestinal tract. Until the late 1950s, silver stains, particularly those identifying endocrine cells, were accompanied by a number of technical difficulties resulting from uncontrolled staining factors. In the 1960s, new silver stains were developed for endocrine cell types and these stains gave reproducible results. One of the "older" silver stains and two of the "newer" ones are emphasized in this presentation, namely the Masson, the Grimelius and the Sevier-Munger techniques. The Masson stain demonstrates the enterochromaffin (EC, serotonin) cells, the Grimelius stain is a broad endocrine cell marker, and the Sevier-Munger technique demonstrates EC and EC-like cells and the C-cells of the thyroid. Especially in the preinummohistochemical era, these staining methods often were used for histopathological diagnosis, particularly the Grimelius technique. The silver stains were developed empirically, and with few exceptions the chemical background is not known. Staining protocols are included.