Chitosan-poly(lactide-co-glycolide) microsphere-based scaffolds for bone tissue engineering: In vitro degradation and in vivo bone regeneration studies
Chitosan-poly(lactide-co-glycolide) microsphere-based scaffolds for bone tissue engineering: In vitro degradation and in vivo bone regeneration studies
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DOI：
10.1016/j.actbio.2010.03.023
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发表时间：
2010-09-01
影响因子：
9.7
通讯作者：
Laurencin, Cato T.
中科院分区：
文献类型：
--
作者：
Jiang, Tao;Nukavarapu, Syam P.;Laurencin, Cato T.
天然聚合物壳聚糖和合成聚合物聚丙交酯乙交酯 (PLACA) 已被研究用于各种组织工程应用。我们之前报道过一种用于承载骨组织工程应用的新型壳聚糖/PLAGA烧结微球支架的制造和体外评估。在本研究中，研究了壳聚糖/PLAGA支架的体外降解特性以及基于壳聚糖/PLAGA的支架在兔尺骨临界尺寸缺损模型中的体内骨形成能力。壳聚糖/PLAGA 支架在体外表现出比 PLAGA 支架更慢的降解。尽管壳聚糖/PLAGA支架在12周的降解期内表现出抗压性能逐渐下降，但抗压强度和抗压模量仍保持在人骨小梁的范围内。基于壳聚糖/PLAGA 的支架能够在兔尺骨临界尺寸缺损模型中引导骨形成。微计算机断层扫描分析表明，使用基于壳聚糖/PLAGA 的支架成功桥接了邻近和远离桡骨两侧的临界尺寸缺损。与壳聚糖/PLAGA 支架相比，肝素和重组人骨形态发生蛋白-2 固定在壳聚糖/PLAGA 支架表面可促进早期骨形成，沿半径的缺损完全桥接，并且机械性能显着增强。此外，组织学分析表明，基于壳聚糖/PLAGA 的支架通过膜内形成支持正常骨形成。 (C) 2010 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
Natural polymer chitosan and synthetic polymer poly(lactide-co-glycolide) (PLACA) have been investigated for a variety of tissue engineering applications. We have previously reported the fabrication and in vitro evaluation of a novel chitosan/PLAGA sintered microsphere scaffold for load-bearing bone tissue engineering applications. In this study, the in vitro degradation characteristics of the chitosan/PLAGA scaffold and the in vivo bone formation capacity of the chitosan/PLAGA-based scaffolds in a rabbit ulnar critical-sized-defect model were investigated. The chitosan/PLAGA scaffold showed slower degradation than the PLAGA scaffold in vitro. Although chitosan/PLAGA scaffold showed a gradual decrease in compressive properties during the 12-week degradation period, the compressive strength and compressive modulus remained in the range of human trabecular bone. Chitosan/PLAGA-based scaffolds were able to guide bone formation in a rabbit ulnar critical-sized-defect model. Microcomputed tomography analysis demonstrated that successful bridging of the critical-sized defect on the sides both adjacent to and away from the radius occurred using chitosan/PLAGA-based scaffolds. Immobilization of heparin and recombinant human bone morphogenetic protein-2 on the chitosan/PLAGA scaffold surface promoted early bone formation as evidenced by complete bridging of the defect along the radius and significantly enhanced mechanical properties when compared to the chitosan/PLAGA scaffold. Furthermore, histological analysis suggested that chitosan/PLAGA-based scaffolds supported normal bone formation via intramembranous formation. (C) 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.